超高效液相色谱-串联质谱法快速测定化妆品中14种性激素

Rapid determination of 14 sexual hormones in cosmetics by ultra high performance liquid chromatography-tandem mass spectrometry

建立了化妆品中14种性激素无基质效应影响的超高效液相-串联质谱分析方法。样品经饱和氯化钠分散后,采用乙腈提取,冷冻离心处理,用乙腈10倍稀释以消除基质效应影响后,以水-甲醇为流动相,梯度洗脱,采用多反应监测进行分析。结果显示,14种性激素在10~200μg/L质量浓度范围内线性关系良好,相关系数(r)均在0.995以上,平均回收率为90.8%~115.2%。本方法准确,灵敏度高,专属性好,检出限低,可用于化妆品中14种性激素的定性及定量测定。

A method of ultra high performance liquid chromatography-tandem mass spectrometry was developed for simultaneous determination of 14 sexual hormones in cosmetics without matrix effects.After the sample was dispersed in saturated sodium chloride,it was extracted with acetonitrile,frozen and centrifuged,and diluted 10 times with acetonitrile to eliminate the matrix effect.The extracts were separated using gradient elution of water and methanol as mobile phase.The analysis of the target compounds was performed under multiple reaction monitoring.The results show that the 14 sexual hormones have a good linearity in the range of 10-200μg/L with correlation coefficients(r)more than 0.995,and the average recoveries of the method are 90.8%-115.2%.This method is accurate,sensitive,specific,low detection limit and can be used for the qualitative and quantitative determinations of 14 kinds of sexual hormones in cosmetics.