粉葛资源产业化过程废弃物中的黄酮类化学成分分析

Analysis of Flavonoids Chemical Components in Waste Material during Industrialization of Pueraria thomsonii Resources

目的:研究粉葛采收及加工过程废弃物中黄酮类化学成分的组成和含量,为该品种资源的综合开发与合理利用提供依据。方法:以江西产粉葛品种“赣葛2号”为对象,采用超高效液相色谱-四极杆-飞行时间质谱和高效液相色谱技术分别检测葛根(带皮或去皮)、葛根皮、葛花、葛须根、葛茎、葛根头、葛根渣(带皮或去皮)以及沉淀葛粉后的工业废水(带皮或去皮)干物质中的黄酮类化学成分组成和含量。结果:所建立的7种黄酮类成分(葛根素、大豆苷、鸢尾黄素-7-O-木糖葡萄糖苷、染料木苷、鸢尾苷、大豆苷元、葛花苷)的含量测定方法的线性、精密度、重复性、稳定性、加样回收率均符合要求。共鉴定出12个黄酮类化学成分,其中葛根、葛根皮、葛茎、葛须根、葛根头、葛根渣和工业废水干物质中的黄酮类成分种类基本一致,主要为葛根素、大豆苷、染料木苷、大豆苷元、丙二酰基黄豆苷元;葛花中主要含有鸢尾黄素-7-O-木糖葡萄糖苷、染料木苷、鸢尾苷、葛花苷、6″-O-木糖基黄豆黄苷,但未检出葛根素、大豆苷及苷元等成分。葛根素含量以葛根头中最高,达5.765%;葛根、葛根渣及废水干物质各带皮样品中葛根素含量均较相应的去皮样品更高。结论:粉葛资源产业化过程的废弃物含有种类丰富、含量较高的黄酮类化合物,可作为获取葛根素等黄酮类化学成分的重要原料资源加以开发利用。

OBJECTIVE:To study the composition and contents of flavonoids chemical components in waste material during industrialization of Pueraria thomsonii resources,and to provide reference for comprehensive development and reasonable utilization of the variety.METHODS:Using“No.2 Gange”of P.thomsonii from Jiangxi as objects,UPLC-Q-TOF-MS and HPLC method were adopted to detect the components and contents of flavonoids in the root(with or without cortex),cortex,flower,fibrous root,stem,head and dregs(with or without cortex)of P.thomsonii as well as dry matter of industrial wastewater(with or without cortex)after precipitation of pueraria powder.RESULTS:The linearity,precision,repeatability,stability and recovery of the established method for content determination of 7 flavonoids(puerarin,daidzin,iridoxine-7-O-xylose glucoside,genistin,iridin,daidzein and kakkalide)were all in line with the requirements.Totally 12 kinds of flavonoids were identified,among which the flavonoids in the root,cortex,stem,fibrous root,head and dregs of P.thomsonii as well as dry matter of industrial wastewater were the same,mainly were puerarin,daidzin,genistein,daidzein and malonyl-daidzein.The flower of P.thomsonii mainly included iridoxine-7-O-xylose glucoside,genistin,iridin,kakkalide,6″-O-xylosyldaidzein,but the components as puerarin,daidzin and its aglycone were not be detected.The content of puerarin in the head of P.thomsonii was the highest(5.765%).The contents of puerarin in root and dregs of P.thomsonii as well as dry matter of industrial waste-water in samples with cortex were all higher than in corresponding peeled sample.CONCLUSIONS:The waste material from the industrialization of P.thomsonii resources contains a lot of flavonoids with rich species and high content,and can be used as an important raw material for obtaining flavonoids such as puerarin.