改良玻璃化冷冻法对小鼠卵母细胞影响的研究

Study on the effect of modified vitrification on the mouse oocytes

目的探讨改良玻璃化冷冻法对小鼠卵母细胞复苏率和解冻后胚胎发育潜能的影响。方法以小鼠卵母细胞为实验材料,分别采用改良和传统玻璃化冷冻方法冻存小鼠卵母细胞,通过解冻后卵子复苏率、受精率、卵裂率及优胚率等分析判断冻存效果。结果改良玻璃化冷冻组的复苏率、卵裂率及优胚率均高于传统玻璃化冷冻组(P<0.05),两组的受精率无显著性差异(P>0.05)。结论相较于传统玻璃化冷冻方法,改良玻璃化冷冻法更有优势,值得推广。

Objective To investigate the effect of modified vitrification freezing on the mouse oocytes. Methods The modified and traditional vitrification freezing methods were used to freeze the mouse oocytes respectively. Then the recovery rates, cleavage rates and optimized embryo rates of the modified and traditional vitrification freezing groups were compared. Results The recovery rates, cleavage rates and optimized embryo rates of the modified vitrification freezing group were all higher than that of the traditional vitrification freezing group(P<0.05), and the fertilization rate of the two groups had no significant difference( P>0.05). Conclusion Compared with traditional vitrification freezing method, improved vitrification freezing method has more advantages and it is worth popularizing.