DDH合并OA患者血清及其滑膜组织中SP、S100A12的表达特点及意义

Expression and Significance of SP and S100A12 in Serum and Synovial Tissue of DDH Patients with OA

目的探讨髋关节发育不良(developmental dysplasia of the hip,DDH)患者血清及其滑膜组织中的P物质(substance P,SP)、钙结合蛋白(S100 calcium binding protein A12,S100A12)表达及其意义。方法本研究采取回顾性研究方法,选取我院2013年4月至2018年4月收治的182例DDH患者,其中单纯的DDH患者114例(DDH组)、DDH合并骨关节炎(osteoarthritis,OA)的患者68例(OA组),同时选取髋部外伤骨折实施手术的40例患者作为对照组,分别采用ELISA法、免疫组化法检测三组的血清及滑膜组织中的SP、S100A12表达情况并进行比较。结果OA组患者的血清SP、S100A12表达水平显著高于DDH组和对照组(P<0.05),DDH组患者的血清SP、S100A12表达水平显著高于对照组(P<0.05);采用ELISA方法检查,OA组患者的髋关节滑膜组织中SP、S100A12表达水平显著高于DDH组和对照组(P<0.05),DDH组患者的髋关节滑膜组织中SP、S100A12表达水平显著高于对照组(P<0.05);采用免疫组化方法检查,OA组患者的髋关节滑膜组织中SP、S100A12表达的OD值高于DDH组和对照组(P<0.05),DDH组患者的髋关节滑膜组织中SP、S100A12表达OD值显著高于对照组(P<0.05)。结论DDH患者血清及髋关节滑膜组织中SP、S100A12表达较正常髋关节滑膜组织显著增高,合并OA的DDH患者SP、S100A12表达的升高更显著,可能与关节退变及关节的炎性改变有关。

Objective To investigate the expression and significance of substance P(SP)and calcium binding protein(S100A12)in serum and synovial tissue of patients with hip dysplasia(DDH).Methods This study takes a retrospective approach.Weselected 182 DDH patients,including 114 DDH patients(DDH group),68 DDH patients with osteoarthritis(OA group),and 40 patients with hip traumatic fracture who underwent surgery as control group.The expression of SP and S100A12 in serum and synovial tissue of the three groups were detected by ELISA and immunohistochemistry and compared.Results The expression levels of SP and S100A12 in serum of OA group were significantly higher than those of DDH group and control group(P<0.05),and the expression levels of SP and S100A12 in serum of DDH group were significantly higher than those of control group(P<0.05).The expression levels of SP and S100A12 in hip synovial tissue of OA group were significantly higher than those of DDH group and control group(P<0.05),and the expression levels of SP and S100A12 in hip synovial tissue of DDH group were significantly higher than those of DDH group(P<0.05).The expression level of S100A12 was significantly higher in OA group than in DDH group and control group(P<0.05).The expression of SP and S100A12 in hip synovium was significantly higher in OA group than in DDH group and control group(P<0.05),and the expression of SP and S100A12 in hip synovium in DDH group was significantly higher than that in control group(P<0.05).Conclusion The expression of SP and S100A12 in serum and hip synovial tissue of DDH patients is significantly higher than that in normal hip synovial tissue.The increase of SP and S100A12 expression in DDH patients with OA is more significant,which may be related to joint degeneration and joints.It is related to inflammatory changes.