三豆饮豆渣膳食纤维提取工艺优化研究

Study on the Optimization of Extraction Process of Dietary Fiber from Sandouyin Soybean Dregs

【目的】以三豆饮豆渣为原料制备膳食纤维,利用单因素试验和响应面法相结合优化制备工艺条件。【方法】通过在酸提单因素试验基础上,采用响应面法以料液比、提取时间、提取温度、提取pH为因素,豆渣膳食纤维含量为响应值,以获得最优酸提工艺。基于最优酸提工艺条件下提取过的豆渣,在碱提单因素试验基础上,采用响应面法以料液比、提取时间、提取温度、提取pH为因素,豆渣膳食纤维含量为响应值,以获得最优碱提工艺。【结果】最优酸提工艺为:料液比(1∶25)、提取时间2.8 h、提取温度87℃、提取pH 4.6。酸提后膳食纤维含量为59%,比原豆渣膳食纤维含量增加14.4%;最优碱提工艺为:料液比(1∶35)、提取时间4 h、提取温度56℃、提取pH 11.8。碱提后膳食纤维含量为75.7%,比原豆渣膳食纤维含量增加30.1%。【结论】经过工艺验证,豆渣中膳食纤维含量实测值和预测值基本一致,该工艺稳定可行,对三豆饮豆渣循环利用具有重要意义。

【Objective】Sandouyin bean dregs was used as raw material to prepare dietary fiber.An single factor test and response surface method were used to optimize the extraction process.【Method】Based on single factor of acid extraction test,by using acid extraction water/solid ratio,acid extraction time,acid extraction temperature and acid extraction pH as response factors,and the extraction content of dietary fiber from bean dregs as response value,response surface method was used to obtain the optimal acid extraction process of dietary fiber.Under the optimum conditions of acid extraction,based on single factor of alkali extraction test,by using alkali extraction water/solid ratio,alkali extraction time,alkali extraction temperature and alkali extraction pH as response factors,and the extraction content of dietary fiber from bean dregs as response value,response surface method was used to obtain the optimal alkali extraction process of dietary fiber from bean dregs.【Result】The optimal acid extraction conditions of dietary fiber from Sandouyin soybean dregs were:the water/solid ratio of 1∶25,acid extraction time of 2.8 h,acid extraction temperature of 87℃and acid extraction pH of 4.6.After acid extraction,the content of dietary fiber was 59%,which increased by 14.4%compared with that of raw bean dregs.The optimal alkali extraction conditions of dietary fiber from Sandouyin soybean dregs were:the water/solid ratio of 1∶35,alkali extraction time of 4h,alkali extraction temperature of 56℃and alkali extraction pH of 11.8.After alkali extraction,the content of dietary fiber was 75.7%,which increased by 30.1%compared with that of raw bean dregs.【Conclusion】In verification process of three batches,the measured values of dietary fiber contents in bean dregs were basically similar with the predicted values,the process in this experiment was stable and feasible,which was of great significance to the recycling of Sandouyin soybean dregs.