枸杞多糖对癫痫大鼠学习记忆能力的影响及抗氧化应激作用

Effects of Lycium Barbarum Polysaccharides on Learning and Memory Ability and Antioxidant Stress in Epileptic Rats

目的:研究枸杞多糖(lycium barbarum polysaccharides,LBP)对癫痫大鼠学习记忆能力的影响及其抗氧化应激作用。方法:将雄性SD大鼠60只随机分为正常对照组、癫痫模型组、LBP干预组,各组20只。正常对照组采取常规饲养,不建立癫痫模型;采用经典氯化锂-匹鲁卡品方法建立癫痫模型,模型组模成模后采取常规饲养;LBP干预组在建立癫痫模型后,采用50 mg/kg LBP灌胃,持续灌胃14 d。14 d后,分别对3组大鼠行morris水迷宫测试。测试完成后,取海马组织,测定海马组织内超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-PX)含量。结果:1)与正常对照组比较,模型组大鼠逃避潜伏期时间明显延长(T 1=7.72,T 2=8.73,T 3=9.92,T 4=11.88;P=0.00);与模型组比较,LBP组大鼠逃避潜伏期时间明显缩短(T 1=5.75,T 2=5.91,T 3=7.80,T 4=6.97,P=0.00)。与正常对照组比较,模型组大鼠平台象限路径百分比与平台象限停留时间明显缩短(T 1=15.05,P=0.00;T 2=7.62,P=0.00);与模型组比较,LBP组大鼠平台象限路径百分比与平台象限停留时间明显延长(T 1=7.62,P=0.00;T 2=2.47,P=0.02)。2)与正常对照组比较,模型组大鼠海马组织SOD、GSH-PX含量明显降低(T SOD=10.16,P=0.00;T GSH=9.03,P=0.00),MDA含量明显升高(T=6.43,P<0.01);与模型组比较,LBP干预组大鼠SOD、GSH-PX含量明显升高(T SOD=4.13,P=0.00;T GSH=5.58,P=0.00),MDA含量明显降低(T=2.50,P=0.02)。结论:LBP能够提高癫痫大鼠学习记忆能力,其机制可能与通过增强抗氧化应激作用保护海马神经元细胞有关。

Objective:To study the effects and possible mechanism of Lycium Barbarum Polysaccharides(LBP)on the learning and memory in epileptic rats.Methods:60 male SD rats were randomly divided into control group,epilepsy model group and LBP group.The control group was fed routinely without establishing epilepsy model.In the model group,the epilepsy model was established by the classic lithium chloride pilocarpine method.After the model was successfully prepared,the normal feeding was adopted.The LBP intervention group was fed with 50 mg/kg LBP for 14 days after establishing epilepsy model.After 14 days all the rats were tested by Morris water maze.After the water maze experiment,the contents of SOD,MDA and GSH-PX were measured in hippocampus of rats.Results:1)The escape latency in the model group was significantly longer than that of the control group(T 1=7.72,T 2=8.73,T 3=9.92,T 4=11.88;P=0.00),and the escape latency in the LBP intervention group was significantly shorter than that of the model group(T 1=5.75,T 2=5.91,T 3=7.80,T 4=6.97,P=0.00).The percentage of plateau quadrant path and plateau quadrant residence time in the model group were significantly shorter than that of the control group(T 1=15.05,P=0.00;T 2=7.62,P=0.00),and the percentage of plateau quadrant path and plateau quadrant residence time in the LBP intervention group were significantly longer than that of the model group(T 1=7.62,P=0.00;T 2=2.47,P=0.02).2)The contents of SOD,GSH-PX in the hippocampus of model group were lower than that of the control group(T SOD=10.16,P=0.00;T GSH=9.03,P=0.00),but the MDA was higher(T=6.43,P<0.01);The contents of SOD,GSH-PX in the hippocampus of the LBP intervention group were higher than that of the model group(T SOD=4.13,P=0.00;T GSH=5.58,P=0.00),but the MDA was lower(T=2.50,P=0.02).Conclusion:LBP can improve the learning and memory ability of epileptic rats,and its mechanism may be the antioxidant stress effect.