杨树HDA902基因在低温胁迫应答反应中的功能

Function of Populus trichocarpa HDA902 Gene in Response to Cold Stress

组蛋白去乙酰化酶在植物非生物胁迫应答反应中具有重要的调控作用。利用RT-PCR的方法从毛果杨中克隆了组蛋白去乙酰化酶基因HDA902。利用农杆菌介导法将其遗传转化到烟草中,并对转基因植株进行低温耐受性分析。研究结果表明,HDA902在烟草中的表达显著提高了转基因株系对低温的耐受性。叶片NBT和DAB染色结果表明,在低温处理后转基因烟草比野生型烟草产生较少的活性氧。丙二醛和脯氨酸含量测定结果表明,在低温条件下,转基因烟草叶片的脯氨酸含量显著高于野生型烟草,而丙二醛含量显著低于野生型烟草。这些研究结果表明,HDA902参与低温胁迫应答反应,其过量表达提高了植株耐低温的能力。

Histone deacetylases plays an important role in plant abiotic stress responses.Histone deacetylase gene HDA902 was cloned from Populus trichocarpa by RT-PCR.The HDA902 gene was transformed into tobacco by Agrobacterium tumefaciens-mediated method.The tolerance of transgenic plants to cold stress was analyzed.The results showed that the expression of HDA902 in tobacco significantly improved the tolerance of transgenic lines to low temperature.The results of NBT and DAB staining showed that transgenic tobacco produced less reactive oxygen species than the wild type after cold treatment.The proline content in the leaves of transgenic plants were much higher than that of wild-type plants,whereas the MDA contents in the leaves of transgenic plants were much lower at low temperature.These results indicated that HDA902 was involved in the response to cold stress,and its overexpression enhanced the tolerance of transgenic plants to cold stress.