细叶百合LpPEX7基因克隆及盐胁迫下的表达特性分析

Cloning of LpPEX7 Gene from Lilium pumilum and Its Expression Characteristics under Salt Stress

从细叶百合的鳞茎中克隆出过氧化物酶体生物合成蛋白基因(LpPEX7),该基因ORF全长957 bp,编码318个氨基酸。LpPEX7蛋白序列包含6个WD40保守结构域,通过同源蛋白序列比对和进化树分析,发现LpPEX7与其他植物的PEX7蛋白具有较高的同源性。LpPEX7基因在细叶百合种子,叶片和鳞茎中的表达量比较高,在根和花中表达量比较低,在H2O2,NaCl,NaHCO3不同逆境处理条件下,LpPEX7基因的表达量都发生了改变。在盐碱和氧化胁迫处理下,LpPEX7过表达拟南芥株系种子的萌发要早于野生型种子的萌发,这些研究结果表明LpPEX7基因与盐碱、氧化逆境有一定的应答关系,为细叶百合的耐盐碱性分子机理研究提供一个非常重要的候选基因。

The peroxidasome biosynthetic gene(LpPEX7)of Lilium pumilum DC was cloned,and its open reading frame length is 957 bp,encoding 318 amino acids.The deduced amino acid sequence of LpPEX7 contains six WD40 conserved domains,through sequence alignment analysis of homologous proteins and evolutionary tree analysis,it was found that LpPEX7 had higher homology with some PEX7 from other plants.The expression of LpPEX7 was higher in seeds,leaves and bulbs,but lower in roots and flowers of L.pumilum.The expression of LpPEX7 changed under H2O2,NaCl and NaHCO3 stress conditions.Subsequently,the seeds germination of LpPEX7 over-expression Arabidopsis thaliana lines as earlier than that of wild seeds under the treatment of salinity and oxidative stress.These results show that the LpPEX7 gene has a certain response relationship with salinity and oxidative adversity.It provides a very important candidate gene for the study of salt-tolerant alkaline molecular mechanism of Lily.