悬钩子属DNA条形码通用序列的初步筛选

Preliminary Selection of Potential DNA Barcodes for Rubus

为了建立悬钩子属(Rubus)植物的DNA条形码分子鉴定技术,筛选获得适用于悬钩子属植物的通用条形码序列。该研究基于GenBank数据对ITS、ITS2、matK、rbcL、trnH-psbA、trnL-trnF 6个DNA条形码序列进行了遗传变异、barcoding gap、建树等评估分析。结果显示,trnH-psbA、matK、rbcL、rtnL-trnF的种内变异与种间变异差异较大,变异分辨率分别为97.32%、83.33%、79.07%、64.95%,存在较大的barcoding gap;NJ一致树分析显示,matK的单系性比例最高(67%),其次为trnH-psbA(64%),rtnL-trnF(43%),rbcL(30%)。结果表明,悬钩子属植物的matK与trnH-psbA序列种内变异与种间变异差异较大,能较好地区分不同物种,具有较大的鉴定潜力。建议将matK和trnH-psbA作为悬钩子属植物鉴定的核心条形码序列,rtnL-trnF、rbcL作为辅助条形码序列。

In order to establish a molecular identification technique of DNA barcoding for Rubus,and screening universal barcode sequences suitable for Rubus.With GenBank data we analyzed the genetic variation,barcoding gap and NJ consensus tree of six DNA barcoding sequences that contain ITS,ITS2,matK,rbcL,trnH-psbA and trnL-trnF.The intraspecific variation and interspecies variation of trnH-psbA,matK,rbcL and rtnL-trnF were different,and the variation resolutions were 97.32%,83.33%,79.07%and 64.95%,respectively.And trnH-psbA,matK,rbcL and rtnL-trnF had obvious barcoding gap;NJ consistent tree showed that matK had the highest proportion of solopathogenicity(67%),and then trnH-psbA(64%),rtnL-trnF(43%),rbcL(30%).The intraspecific variation and interspecies of matK and trnH-psbA sequences for Rubus are different,which can distinguish different species well with great identification potential.It is recommended to use matK and trnH-psbA as the core barcode sequences identified for Rubus,and rtnL-trnF and rbcL are used as auxiliary barcode sequences.