板栗贮藏期真菌群落结构分析初报

Preliminary Study of Chestnut Fungus Community During Storage

为探明板栗贮藏期真菌群落结构的种质间差异性,采用高通量测序手段对6种板栗的栗壳和栗仁进行真菌ITS扩增子测序。分析发现不同种质板栗的主要病原菌差异较大,祝阳镇麻塔村古栗病原菌未大量检出,祝阳镇桐花沟村古栗为赤霉菌,黄前镇大峪村古栗为镰孢菌和葡萄座腔菌,下港镇砖瓦窑古栗为青霉菌,角峪镇洪河村古栗为木霉,燕山早丰板栗为青霉菌和炭疽菌。α-多样性指数分析发现,祝阳镇麻塔村古栗的真菌多样性、优势度、菌群丰度和系统发育多样性均较高。种质内比较发现,祝阳镇麻塔村古栗栗壳的镰孢菌丰度显著低于栗仁;桐花沟古栗栗壳的赤霉菌丰度显著低于栗仁;角峪镇洪河村古栗栗壳的青霉菌丰度显著高于栗仁;燕山早丰品种栗壳的青霉菌丰度显著高于栗仁,炭疽菌属真菌丰度显著低于栗仁。同时,祝阳镇麻塔村古栗、黄前镇大峪村古栗、桐花沟古栗和燕山早丰板栗的栗壳和栗仁的真菌群落结构相似度高;角峪镇洪河村古栗和下港镇砖瓦窑古栗栗壳和栗仁的真菌群落结构差异较大。

To verify the diversity of fungal community structure in chestnut during storage,the fungal ITS amplitons of chestnut shell and pip from 6 species was sequenced by high-throughput method.It was found that the main pathogen of different chestnut variety differed greatly.Few were found in ancient chestnut in Mata village,Zhuyang town.Ancient chestnut in Tonghuagou village,Zhuyang town was Gibberella.Ancient chestnut in Dayu village,Huangqian town was Fusarium and Botryosphaeria.Ancient chestnut in Zhuanwayao,Xiagang town was Penicillium.Ancient chestnut in Honghe village,Jiaoyu town was Trichoderma.Castanea mollissima cv.Zaofeng was Penicillium and Colletotrichum.Community diversity index analyze showed that,fungus diversity,dominance,abundance and phylogenetic diversity of ancient chestnut in Mata village were the highest.Analysis within species found that,for ancient chestnut in Mata village,zhuyang town,the abundance of Fusarium in pip was significantly lower than that in shell.For ancient chestnut in Tonghuagou village,Zhuyang town,the abundance of Gibberella in pip was significantly lower than that in shell.For ancient chestnut in Honghe village,Jiaoyu town,the abundance of Penicillium in pip was significantly higher than that in shell.For C.mollissima cv.Zaofeng,the abundance of Penicillium in pip was significantly higher than that in shell,and the abundance of Colletotrichum was significantly lower than that in shell.At the same time,the fungal community structure of chestnut pip and shell was highly similar for ancient chestnut in Mata village,Zhuyang town,ancient chestnut in Dayu village,Huangqian town and C.mollissima cv.Zaofeng;and the fungal community structure of chestnut pip and shell differed greatly for ancient chestnut in Honghe village,Jiaoyu town and ancient chestnut in Zhuanwayao,Xiagang town.