系统性红斑狼疮患者腺嘌呤-胸腺嘧啶碱基富含互作域3a^+B细胞的变化及临床意义

The clinical significance of AT-rich interaction domain 3a^+B cell change in patients with systemic lupus erythematosus

目的探讨转录因子——腺嘌呤-胸腺嘧啶碱基富含互作域3a(ARID3a)在SLE患者外周血B细胞中的表达及临床意义。方法流式细胞术检测17例SLE患者、13名健康对照外周血中ARID3a+B细胞的比例,结果采用独立样本t检验比较,并采用Pearson相关性分析法分析ARID3a+B细胞的比例与临床指标的相关性;选取14例LN患者肾脏穿刺蜡块,采用免疫组织化学方法检测肾脏ARID3a的表达量。结果SLE患者外周血ARID3a+B细胞比例[(51.6±3.2)%]显著高于健康对照[(32.6±3.4)%](t=4.0,P<0.01);ARID3a+B细胞比例与尿蛋白定量(24 h)(r=0.68,P<0.05)呈正相关;活动性LN患者外周血ARID3a+B细胞比例[(62.3±4.3)%]显著高于非活动性LN和无肾脏损害SLE患者[(43.3±2.8)%](t=3.8,P<0.01);LN患者肾小球及肾小管细胞ARID3a的表达量显著升高。结论B细胞过度表达转录因子ARID3a可能参与SLE患者LN的发病,可作为预测狼疮疾病发展的新指标。

ObjectiveTo investigate the expression of transcription factor AT-rich interaction domain 3a(ARID3a)in peripheral blood B cells of patients with systemic lupus erythematosus(SLE)and its clinical significance.MethodsPeripheral blood mononuclear cells were isolated from 17 SLE patients and 13 healthy controls.Then,the expression of ARID3a by B cells was determined by flow cytometry.Data was analyzed with independent sample t test.The correlations between the frequencies of ARID3a+B cells and clinical indicators of SLE patients were assessed by Pearson cor relation analysis.The expression of ARID3a in kidney was detected by immunohistochemistry in 14 cases of lupus nephritis(LN).ResultsThe percentages of ARID3a+B cells in SLE patients[(51.6±3.2)%]were significantly higher than those in healthy controls[(32.6±3.4)%](t=4.0,P<0.01).There was a positive correlation between the percentages of ARID3a+B cells in SLE patients and their 24-hour urinary protein(r=0.68,P<0.05).Furthermore,the percentages of ARID3a+B cells in peripheral blood from patients with active LN[(62.3±4.3)%]were remarkably higher than that from patients without LN or with inactive LN[(43.3±2.8)%](t=3.8,P<0.01).The expression of ARID3a in glomerula and tubules of LN patients markedly increased.ConclusionElevated expression of transcription factor ARID3a in B cells may participate in the pathogenesis of LN.