基于高通量测序分析蟹糊微生物菌群多样性

Analysis of Microbial Community Diversity of Crab Paste by High-Throughput Sequencing

为探索不同贮藏温度下蟹糊微生物菌群结构,应用Illumina MiSeq高通量测序技术研究蟹糊在-20℃和4℃贮藏3 d后的微生物菌群多样性。结果表明:-20℃贮藏3 d的蟹糊样品V1-V3和V3-V4区域扩增所得微生物菌属分别为18属和26属,4℃贮藏3 d的蟹糊样品V1-V3和V3-V4区域扩增所得微生物菌属分别为16属和19属。相对丰度大于1%的菌属为肉食杆菌属(Carnobacterium)、Jeotgalibaca、环丝菌属(Brochothrix)、葡萄球菌属(Staphylococcus)、嗜冷杆菌属(Psychrobacter)、弧菌属(Vibrio)。-20℃贮藏3 d的蟹糊样品优势菌属为肉食杆菌属(V1-V3,68.81%;V3-V4,69.29%)和弧菌属(V1-V3,16.75%;V3-V4,14.39%)。4℃贮藏3 d的蟹糊样品优势菌属为肉食杆菌属(V1-V3,73.37%;V3-V4,70.35%)、Jeotgalibaca(V1-V3,11.82%;V3-V4,12.35%)和葡萄球菌属(V1-V3,5.45%;V3-V4,5.01%),同时Jeotgalibaca和葡萄球菌属相对丰度显著高于-20℃贮藏的蟹糊样品。以上结果表明,蟹糊在不同贮藏温度下菌群多样性、优势菌属及其相对丰度均存在显著差异(P<0.05)。本研究为有效预防腌制生食蟹糊食源性疾病的发生及控制技术的开发提供理论依据。

In order to explore the microbial community structure of crab paste stored at different temperatures,the microbial diversity before and after 3 days of storage at–20 or 4℃were investigated by using Illumina MiSeq high-throughput sequencing technique.The results showed that a total of 18 and 26 genera of microorganisms from crab paste samples stored at-20℃for 3 days,as well as 16 and 19 genera from those stored at 4℃for 3 days were obtained through amplification of the 16S rRNA V1-V3 and V3-V4 regions,respectively.The main genera with relative abundance over 1%were Carnobacterium,Jeotgalibaca,Brochothrix,Staphylococcus,Psychrobacter and Vibrio.The dominant genera at–20℃were Carnobacterium(V1-V3,68.81%;V3-V4,69.29%)and Vibrio(V1-V3,16.75%;V3-V4,14.39%).The dominant genera at 4℃were Carnobacterium(V1-V3,73.37%;V3-V4,70.35%),Jeotgalibaca(V1-V3,11.82%;V3-V4,12.35%)and Staphylococcus(V1-V3,5.45%;V3-V4,5.01%).Meanwhile,the relative abundances of Jeotgalibaca and Staphylococcus at 4℃were markedly higher than those at–20℃.These results indicated that the microbial diversity,dominant genera and their abundances in crab paste varied significantly with storage temperature(P<0.05).This study provides a theoretical basis for the prevention and control of foodborne diseases caused by pickled raw crab paste.