谷子Trithlix转录因子鉴定与生物信息学分析

Bioinformatics Identification and Analysis of the Trihelix Transcription Factor Family in Setaria italica

Trihelix转录因子家族在植物生长发育、生物胁迫和非生物胁迫等方面具有重要的作用,但是目前关于谷子Trihelix转录因子家族研究鲜见报道。本试验共鉴定到34个谷子转录因子,对其进行染色体定位、系统进化树、保守基序、基因结构和不同组织的表达分析发现,该家族成员均含有高度保守的Trihelix结构域,分为5个亚族,同一亚族含有相同的保守基序,同一亚族含有相似的基因结构,在不同的亚族中,组织表达模式不同。本研究初步明确了谷子Trihelix转录因子家族成员进化关系和结构特点,为进一步研究谷子Trihelix转录因子家族的系统发育以及生物学功能奠定了基础,为谷子的分子育种提供科学依据。

Trithlix transcription factor family plays an essential role in plant growth,development and biotic and abiotic stress response,but the studies about this Setaria italica Trihelix transcription factor family not have been reported.In the study,34 members of Trihelix family were identified in Setaria italica.The chromosome location,phylogenetic tree,conserved motif,genes structure and the expression in different tissues were analyzed.The results indicated that 34 SiTrihelix genes contain highly conserved and Trithlix domain,and were divided into five main groups(GT-1,GT-2,GTγ,SH4 and SIP1),Trihelix transcription factor contains the same conserved motif in the same subgroup,and the gene structure is also similar,in addition,tissue expression pattern was different in different subgroup.Our results preliminarily identify the evolution and structure characterization,which will provide a basis to further reveal the molecular evolution and biological function of the Trihelix transcription factor family.