姜黄提取物对运动小鼠生理疲劳和生理机能的影响

Effects of Turmeric Extract on Physiological Fatigue and Physiological Function of Exercise Mice

为了考察姜黄提取物姜黄素对运动小鼠生理疲劳和生理机能的影响,本研究将ICR雄性小鼠随机分成4组,即对照组、低剂量组、中剂量组和高剂量组,每组10只。低、中和高剂量组小鼠异常按照10 mg/kg、20 mg/kg和50 mg/kg的剂量每天灌胃小鼠,对照组小鼠每天灌胃等体积的生理盐水。4组小鼠连续灌胃28 d,然后按照5%体重的恒定负荷进行负重游泳运动,并记录力竭时间。并检测游泳后各组小鼠血清中的乳酸、氨、血糖、血尿素氮(BUN)、肌酸激酶(CK)、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平。另外,检测各组小鼠肝脏和肌肉组织中的糖原水平。研究显示,高剂量组的力竭时间与对照组相比显著提高了72.66%(p<0.05)。与对照组相比,高剂量组的乳酸水平显著降低了41.33%,高剂量组的血氨水平显著降低了50.33%,高剂量组的血糖水平显著降低了29.65%,高剂量组的BUN水平显著降低了36.33%(p<0.05)。与对照组相比,高剂量组的CK水平显著降低了51.13%,高剂量组的AST水平显著降低了32.97%,高剂量组的ALT水平显著降低了37.68%(p<0.05)。与对照组相比,高剂量组的肌糖原水平显著升高了50.77%(p<0.05)。然而,补充姜黄素对小鼠肝糖原水平未见明显影响(p>0.05)。研究结论初步表明,补充姜黄素可有效降低机体的疲劳相关指标和运动损伤指标,提高肌糖原水平,从而改善机体的生理机能,发挥抗疲劳作用。

In order to investigate the effects of turmeric extract(curcumin) on the physiological fatigue and physiological function of exercised mice, in this study, ICR male mice were randomly divided into 4 groups, namely,control group, low-dose group, middle-dose group and high-dose group, with 10 mice in each group. Mice in the low, medium and high dose groups were administrated daily at doses of 10 mg/kg, 20 mg/kg and 50 mg/kg, and control mice were given an equal volume of normal saline daily. Four groups of mice were intragastrically administered for 28 days, then subjected to weight-bearing swimming exercise at a constant load of 5% body weight, and the exhaustion time was recorded. The levels of lactic acid, ammonia, glucose, blood urea nitrogen(BUN), creatine kinase(CK), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in the serum of the mice after swimming were detected. In addition, glycogen levels in liver and muscle tissues of each group of mice were examined. Studies have shown that the exhaustion time of the high-dose group was significantly increased by 72.66% compared with the control group(p<0.05). Compared with the control group, the lactic acidlevel in the high-dose group was significantly reduced by 41.33%, the blood ammonia level in the high-dose group was significantly reduced by 50.33%, the blood glucose level in the high-dose group was significantly reduced by 29.65%, and the BUN level in the high-dose group was significantly reduced by 36.33%(p<0.05).Compared with the control group, the CK level in the high-dose group was significantly reduced by 51.13%, the AST level in the high-dose group was significantly reduced by 32.97%, and the ALT level in the high-dose group was significantly decreased by 37.68%(p<0.05). Compared with the control group, the muscle glycogen level in the high-dose group was significantly increased by 50.77%(p<0.05). However, supplementation with curcumin had no significant effect on liver glycogen levels in mice(p>0.05). Preliminary findings showed that supplementation of curcumin can effe-ctively reduce the body’s fatigue-related indicators and sports injury indicators, improve muscle glycogen levels, thereby improving the body’s physiological functions and exerting anti-fatigue effects.