两种流式细胞仪检测CD4^+T淋巴细胞结果比较分析

Comparison of CD4^+T lymphocytes measured by using two types of flow cytometry instruments

目的比较2种不同型号流式细胞仪检测CD4^+T淋巴细胞绝对数的一致性。方法对现场收集的220例人类免疫缺陷病毒(HIV)感染者/艾滋病(AIDS)患者全血样本进行CD4^+T淋巴细胞绝对数检测,分析深圳迈瑞公司BricyteE6和美国BD公司FACSClibur 2种流式细胞仪间及不同抗体组合检测结果的差异。结果随机选择的30例样本检测结果显示BricyteE6型流式细胞仪批间重复性较好。用FACSClibur流式细胞仪CD45/CD3/CD4/CD8四色抗体、CD3/CD4/CD8三色抗体及BricyteE6流式细胞仪CD45/CD3/CD4/CD8四色抗体分别对70例全血样本进行检测,结果显示CD4^+T淋巴细胞绝对数分别为(477.65±276.40)、(479.73±241.45)和(472.92±259.70)个/μl,表明不同仪器的三色抗体或四色抗体组合试剂检测结果差异无统计学意义(P>0.05)。对120例样本进行批量检测及不同范围的结果分析,结果显示2种流式细胞仪检测结果分别为(459.85±226.24)个/μl、(435.54±222.78)个/μl,一致性较高。结论2种流式细胞仪检测CD4^+T淋巴细胞绝对数一致性较好,三色抗体组合可满足检测需求。

Objective To compare the consistency of CD4^+T lymphocytes measured by two types of flow cytometry.Methods A total of 220 blood samples of HIV/AIDS were detected for CD4^+T lymphocyte by using two types of cytometry instruments,the Mindray BricyteE6 and the BD FACSClibur.Different antibody combinations were analyzed and compared.Results BricyteE6 showed good repeatability for 30 samples;70 samples were detected separately by CD3/CD4/CD8,CD45/CD3/CD4/CD8 of FACSClibur,and CD45/CD3/CD4/CD8 of BricyteE6.The absolute numbers of CD4^+T lymphocytes were(477.65±276.40),(479.73±241.45),and(472.92±259.70)cells/μl,respectively.There were no statistically significant difference between the two different instruments,three-color antibody or four-color antibody detections.The average of CD4^+T lymphocytes of the 120 samples detected by the two instruments were(459.85±226.24)and(435.54±222.78)cells/μl,respectively,indicating high consistency in the detection of CD4^+T lymphocytes by two different types of flow cytometry.Conclusions Two types of BricyteE6 and FACSClibur flow cytometry demonstrated high consistency for detecting CD4^+ T lymphocytes and the three-color antibody can satisfy the detecting needs.