摘要
目的探讨靶向性近红外荧光探针(靶向MMP和RGD)在小鼠结肠癌模型中的应用。方法人结肠癌细胞HCT116和SW620分别接种于裸鼠皮下。接种后3周,尾静脉注射两种近红外探针,在6 h、24 h和48 h分别进行荧光光学成像,观察靶向性近红外荧光探针在小鼠体内的分布情况。结果两种靶向性近红外荧光探针都能特异性结合裸鼠结肠癌移植瘤的不同成分,同样的结肠癌细胞形成的移植瘤包括多种疾病标志物。这些疾病标志物分布在同一个肿瘤结节的不同部位。探针注射后6 h就能清晰显影,直到48 h仍然可见信号。结论MMP探针和RGD探针可以用来动态监测小鼠体内结肠癌细胞生长情况。非侵入性的多靶点光学探针为同时研究结肠癌组织不同成分提供了有效的工具。
Objective To study the application of target-specific near-infrared fluorescent probes(targeting MMP and RGD) in mouse colon cancer model. Methods HCT116 and SW620 colon cancer xenograft models were established through subcutaneous injection in BALB/c nude mice. Imaging studies were performed at 6 h, 24 h and 48 h in colon cancer xenograft models 3 weeks after subcutaneous inoculation. RGD and MMP probes were imaged on tumor-bearing mice using multi-wave length optical imaging modalities.The distribution of MMP and RGD probes in mice was observed. Results Image data demonstrated that each agent bound to a specific disease target component. The same colon cancer xenograft contained multiple disease markers. Those disease markers were heterogenetically distributed in the same tumor nodule. The molecular imaging probes had different distributions in the whole body and inside the tumor nodule. All target-specific probes yielded high tumor-to-background ratios from 6 h to 48 h after injection. Conclusion Target-specific molecular imaging probes can be used to study colon cancer in vivo. Noninvasive multi-target-specific molecular imaging probes could provide tools to simultaneously study multiple colon cancer components.
作者
李强
邹臣丹
冯亮
汤庆超
邹朝霞
LI Qiang;ZOU Chen-dan;FENG Liang;TANG Qing-chao;ZOU Chao-xia(Department of General Surgery,The Second Affiliated Hospital of Harbin Medical University;Department of Biochemistry and Molecular Biology,Harbin Medical Univeristy,Harbin 150081,China)
出处
《哈尔滨医科大学学报》
CAS
2019年第4期337-341,共5页
Journal of Harbin Medical University
基金
黑龙江省教育厅科学技术研究项目(12531313)
关键词
结肠癌
近红外荧光探针
活体成像
基质金属蛋白酶
RGD
colon cancer
near-infrared fluorescent probe
in vivo imaging
matrix metalloproteinase
RGD