流式细胞术在非霍奇金淋巴瘤患者中的诊断价值

Application value of flow cytometry in diagnosis of patients with non-Hodgkin’s lymphoma

目的探讨流式细胞术(FCM)在非霍奇金淋巴瘤(NHL)中的诊断价值。方法采用FCM对51例外周血或骨髓血中出现淋巴细胞或异常淋巴细胞增多的NHL患者进行检测,总结NHL的免疫表型特征,以协助临床诊断。结果51例NHL患者中,男30例,女21例;B细胞淋巴瘤44例,T/NK细胞淋巴瘤7例;FCM免疫表型可鉴别小B细胞淋巴瘤,且对于慢性淋巴细胞白血病/小淋巴细胞淋巴瘤(CLL/SLL)、毛细胞白血病(HCL)、T大颗粒淋巴细胞白血(T-LGLL)、NK细胞-慢性淋巴增殖性疾病(NK-CLPD)通过FCM可确诊。结论流式细胞术是伴有血液或骨髓侵犯NHL诊断和鉴别诊断的重要方法,在NHL的诊断、分类及亚型判断中具有重要价值。

Objective To discuss the application value of flow cytometry in diagnosis of patients with non-Hodgkin’s lymphoma(NHL). Methods FCM was used to detect 51 NHL patients with lymphocyte or abnormal lymphocyte proliferation in peripheral blood or bone marrow, the immunophenotypic characteristics of NHL were summarized to assist clinical diagnosis. Results Among the 51 NHL patients, 30 were males and 21 were females;44 cases of B-cell lymphoma and 7 cases of T/NK cell lymphoma. Immunophenotype identified small B-cell lymphoma, and CLL/SLL, HCL, T-LGLL, and NK-CLPD was diagnosed by FCM. Conclusion Flow cytometry is an important method for the diagnosis and differential diagnosis of NHL with blood or bone marrow involvement. Flow cytometry provided the valuable in the diagnosis, classification and subtype judgment of NHL.