碱性双氧水预处理玉米秸秆性质研究

Investigation of Alkaline Hydrogen Peroxide Characteristic in Corn Straw Pretreatment

使用碱性双氧水对玉米秸秆进行预处理,可以有效提高秸秆的酶解效果。实验表明,最优预处理参数为使用分别占秸秆质量16%的H2O2和25.6%的NaOH,于40℃下预处理秸秆24h。对经不同预处理剂处理后的秸秆进行酶解,发现NaOH及碱性双氧水预处理秸秆的酶解还原糖产量为7.48 g/L和8.26 g/L,而经H2O及H2O2预处理秸秆的还原糖产量仅为1.35g/L和1.59g/L。通过木质纤维素含量及SEM分析发现,氢氧化钠主要作用为溶解秸秆中的木质素及半纤维素,而双氧水的存在则会破坏秸秆表面结构。计算秸秆预处理前后质量损失发现,双氧水的存在不能显著提高秸秆预处理后的质量损失,但会氧化分解被氢氧化钠溶解的大分子物质,对此过程机理及产物还需进行深入的研究。

The use of high alkaline hydrogen peroxide could dramatically enhance the enzymolysis of corn straw. The results showed that the optimal experiment condition was conducting straw with H2O2 and NaOH accounting 16% and 25.6% of the corn straw mass, respectively, at 40°C for 24 h. Enzymolusis of the straw pretreated by different agents showed the reducing sugar of corn straw pretreated by NaOH and alkaline hydrogen peroxide were 7.48 g/L and 8.26 g/L, respectively, while the reducing sugar of H2O and H2O2 pretreatment was only 1.35 g/L and 1.59 g/L. SEM and lignocellulose analysis reviewed that the lignin and cellulose in straw were mainly dissolved by NaOH and the surface structure of straw mainly destroyed by H2O2. The calculation of straw mass before and after pretreatment showed, although the present of H2O2 was not able to significantly enhance the mass loss of pretreated straw, it could decompose the macromolecule dissolved by NaOH. Therefore, the reaction mechanism and its productions should be further studied.