摘要
CRISPR/Cas9系统通过靶向突变对植物基因组进行编辑,是研究基因功能和作物改良的第三代基因编辑工具。本文介绍了CRISPR/Cas9打靶系统基本结构、工作原理,并展示了其在作物基因功能研究和品质改良中的应用前景。
CRISPR/Cas9 system is a third generationtool for gene function studying and crop improvement by modified plants genome with the multiple targeted mutations.In this review,the basic structure and principle of genome editing CRISPR/Cas9 system and its applications in gene functional research and precision molecular breeding of crops were shown.
作者
宋丽莉
黄艳娜
蒋玮
郑洪建
赵子轶
唐雪明
SONG Lili;HUANG Yanna;JIANG Wei;ZHENG Hongjian;ZHAO Ziyi;TANG Xueming(Biotech Research Institute,Shanghai Academy of Agricultural Sciences,Supervision and Test Center for Ecological Environment Safety of Crops of MOA(Shanghai),Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China;Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,CIMMYT-China Specialty Maize Research Center,Shanghai 201403,China;Shanghai Ronghui Biotechnology Company Limited,Shanghai 200231,China)
出处
《上海农业学报》
2020年第1期150-154,共5页
Acta Agriculturae Shanghai
基金
上海市种业发展项目[沪农科创字(2018)第1-3号]
上海市重点攻关项目[沪农科攻字(2015)第6-1-5号]
上海市玉米产业技术体系[沪农科产字(2017)第10号]
上海农科院卓越团队项目[农科创2017(B-07)]
上海市领军人才项目(2017)
上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-34号]
上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-32号]。
