腹腔镜结直肠癌手术治疗对结直肠癌患者血清中miR-17、miR-21、miR-20a和miR-92水平的影响

Analysis of the effect of laparoscopic colorectal cancer surgery on serum levels of miR-17, miR-21, miR-20a and miR-92 in patients with colorectal cancer

目的探究腹腔镜结直肠癌手术对结直肠癌(CRC)患者血清miR-17、miR-21、miR-20a和miR-92水平的影响。方法选取2015年6月-2018年6月该院收治的CRC患者120例作为研究对象,采用实时荧光定量PCR反应(RT-qPCR)检测血清中miR-17、miR-21、miR-20a和miR-92水平;使用生活质量核心调查问卷第3版(QLQ-C30)评估腹腔镜结直肠癌手术治疗前后患者的状况。结果经腹腔镜结直肠癌手术治疗后,CRC患者的躯体、认知、情绪、角色和社会功能评分均得到明显提高;患者血清中miR-17、miR-21、miR-20a和miR-92的相对水平均明显下降;通过相关性分析发现,miR-17、miR-20a和miR-92的相对量变化与QLQ-C30评分变化有相关性(P<0.05),而miR-21则无相关性(P>0.05)。结论腹腔镜结直肠癌手术治疗后,CRC患者血清中miR-17、miR-20a和miR-92的水平可以作为评估治疗效果的潜在标志物。

Objective To investigate the effects of laparoscopic colorectal cancer surgery on serum miR-17,miR-21,miR-20a and miR-92 levels.Methods 120 patients with colorectal cancer from June 2015 to June 2018 were enrolled in the study.Real-time quantitative PCR(RT-qPCR)was used to detect miR-17,miR-21,miR-20a and miR-92 levels in serum.The QLQ-C30 score was used to assess the condition of patients before and after laparoscopic colorectal surgery.Results After laparoscopic colorectal cancer surgery,the body score,cognitive score,emotional score,role score and social function score were significantly improved;The miR-17,miR-21,miR-20a and miR-92 in serum decreased significantly.Correlation analysis showed that the relative changes of miR-17,miR-20a and miR-92 were correlated with the change of QLQ-C30 score(P<0.05),while miR-21 was not(P>0.05).Conclusion The serum level of miR-17,miR-20a and miR-92 in patients with laparoscopic colorectal cancer can be used as a potential marker to evaluate the therapeutic effect.