新型体外细胞加压培养装置的构建及应用

Development and application of a new pressure loading apparatus for cells in vitro

目的:设计一种新型简易细胞加压培养装置模拟在体外培养条件下对细胞施加流体静压,为进一步研究恒定流体静压条件下体外细胞变化打下基础。方法:装置包括压力培养罐、微压传感器、可编程序控制器以及微型气泵。通过在可编程序控制器上编写控制程序来控制微型气泵的运行,进而调节培养罐内的气体压力,从而控制细胞受到的流体静压。结果:装置的压力加载范围为0~120 mmHg,波动幅度为±2 mmHg。可将常规细胞培养板及培养瓶平稳放置在压力培养罐中并施加预设的流体静压。结论:该装置具有良好的准确性与可靠性,可用于在体外研究恒定流体静压下细胞变化。

Objective To design a new pressure loading apparatus for cells in vitro for simulating the hydrostatic pressure on cells in vitro,thereby providing a foundation for the further study on the changes of cells in vitro under hydrostatic pressure.Methods The designed apparatus consisted of a pressure chamber,a micropressure sensor,a programmable controller and a mini air pump.The running status of the mini air pump was regulated by the control program coded on the programmable controller,so that the hydrostatic pressure on cells was controlled by regulating the gas pressure inside the chamber.Results The loading pressure of the apparatus ranged from 0 to 120 mmHg,with a fluctuation range of±2 mmHg.The common cell culture flask or plate could be steadily put in the pressure chamber and loaded with preset hydrostatic pressure.Conclusion The designed pressure loading apparatus has high accuracy and reliability,and it is suitable for studying the effects of constant hydrostatic pressure on cells in vitro.