偃松松塔多糖的单糖组成分析及活性研究

Monosaccharide Composition and Biological Activity of Polysaccharides from Pine Cones of Pinus pumila (Pall.) Regel

偃松松塔经乙醇提取后,渣经热水提取、喷雾干燥、醇沉制备偃松松塔多糖(PPCP),并用苯酚硫酸法检测多糖的含量,气相色谱法测定PPCP的单糖组成。研究结果表明:PPCP是一种杂多糖,主要由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成,具有典型的多糖红外光谱特征,4种单糖物质的量比为2.33∶1.00∶2.20∶1.94。通过测定PPCP清除DPPH·、·ABTS^+的能力以及还原能力评价PPCP抗氧化活性,然后通过体外实验评估其对RAW 264.7巨噬细胞免疫调节活性,结果表明:偃松松塔多糖有较强的自由基清除能力和Fe^3+还原能力,偃松松塔多糖以浓度依赖性方式表现出较强的抗氧化活性,在多糖质量浓度为2.0 g/L时,DPPH·的清除率(79.72%)达到最大,·ABTS^+清除率(39.63%)达到最大,其Fe^3+还原能力也达到最大值(0.78);松塔多糖能够刺激RAW 264.7巨噬细胞产生大量的NO,并没有对细胞增殖产生影响。

The pine cones of Pinus pumila(Pall.)Regel were extracted by ethanol.From the resulted residues,the polysaccharides of P.pumila cones(PPCP)were obtained by hot water extraction,spray drying and alcohol precipitation processes.The content of the polysaccharides was determined by phenol sulfuric acid method.The monosaccharide composition analysis of the polysaccharide conducted by gas chromatography revealed that it was a heteropolysaccharide and consisted of L-arabinose,D-mannose,D-glucose and D-galactose in the molar ratio of 2.33∶1.00∶2.20∶1.94,which indicated typical IR characteristics of polysaccharides.The antioxidant activity of polysaccharides was evaluated by DPPH free radical(DPPH·)scavenging capacity,ABTS free radical(·ABTS^+)scavenging capacity and reducing capacity.The results showed that pine cones polysaccharides of P.pumila exhibited significant scavenging effects on DPPH·,·ABTS^+ and strong reducing power in a concentration-dependent manner.The maximum scavenging rate of polysaccharides against DPPH·and·ABTS^+ were 39.63% and 39.63%,and the maximum reducing capacity was 0.78 at the concentration of 2.0 g/L.In addition,polysaccharides of P.pumila cones could stimulate RAW 264.7 macrophages to produce a large amount of NO,but had no effect on cell proliferation.