基于SSR的藕莲新品种(系)指纹图谱构建

Construction of Fingerprint Map for Root Lotus Varieties(Lines) by SSR

[目的]利用SSR分子标记构建藕莲品种(系)鉴定的DNA指纹图谱,为藕莲品种鉴定和新品种选育提供技术支持。[方法]利用SSR标记对9个莲藕新品种(系)进行鉴定,统计条带并进行遗传多样性分析,选择核心引物,建立DNA指纹图谱。[结果]从45对引物中筛选到9对多态性引物,对9个品种(系)扩增得到条带,共扩增出52条带,其中差异条带32条,多态性比率为61.54%,扩增片段大小为100~400 bp。选出4对SSR核心引物构建9个藕莲品种(系)的DNA指纹图谱。[结论]藕莲品种遗传多样性水平较低,4对引物组合所构建的DNA指纹图谱可用于藕莲品种鉴定。

[Objective]With the aim to identify root lotus varieties,SSR molecular markers were used to get its DNA fingerprint map.[Method]SSR primers were screened out,the PCR and polyacrylamide gel electrophoresis were carried out for the 9 root lotus varieties,genetic diversity analysis were performed,and the core primers were selected to set up an identified fingerprint system.[Result]9 pairs of primer with clear band and high polymorphism were selected from 45 pairs of primer.A total of 52 bands were amplified from root lotus varieties,32 bands among them were polymorphic,the percentage of polymorphism band(PPB)was 61.54%.Four core primers were selected out to set up fingerprint of 9 root lotus varieties.[Conclusion]The genetic diversity of root lotus varieties was low,DNA fingerprint map constructed by 4 pairs of primer combinations can he used in the identification of root lotus varieties.