摘要
设计合成了一种基于香豆素衍生物的"关-开"型荧光探针CO-NBS,用于选择性识别生物硫醇,包括半胱氨酸(Cys)、高半胱氨酸(Hcy)和谷胱甘肽(GSH).采用紫外-可见吸收光谱和荧光光谱对该探针的光学性能进行研究,实验结果表明,该探针能够实现对Cys、Hcy和GSH快速、高灵敏性和高选择性的检测,检测限分别为92、30、62 nmol/L.此外,该探针具有低细胞毒性和良好的生物相容性,能够成功应用于活细胞和斑马鱼中的生物硫醇的荧光成像.
An "off-on" fluorescent probe CO-NBS based coumarin derivative was designed and synthesized, which can respond selectively with biothiols including cysteine(Cys), homocysteine(Hcy) and glutathione(GSH). The optical properties were studied by UV-Vis absorption spectrum and fluorescence spectrum. The results showed that probe was capable to respond rapidly, sensitively and selectively to Cys, Hcy and GSH with the detection limits of 92, 30 and 62 nmol/L, respectively. In addition, with low cytotoxicity and good biocompatibility, the probe can be successfully applied to image biothiols in living cells and in living zebrafish.
作者
周婷婷
杨瑜涛
周柯岩
胥稳智
李玮
ZhouTingting;Yang Yutao;ZhouKeyan;Xu Wenzhi;Li Wei(College of Chemistry&Environmental Science,Hebei University,Baoding 071002;Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education,Hebei University,Baoding 071002)
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2019年第12期3498-3504,共7页
Chinese Journal of Organic Chemistry
基金
国家自然科学基金(No.21807021)、河北大学药物化学与分子诊断教育部重点实验室提升计划(No.ts2019004)、河北大学高层次引进人才资助项目
河北省自然科学青年基金(No.B2017201093)~~
关键词
荧光探针
香豆素衍生物
生物硫醇
荧光成像
fluorescent probe
coumarin derivatives
biothiols
fluorescence imaging
