基于功能微拓扑结构的细胞定位培养芯片

Cell Positioning Culture Chip Based on Functional Micro Topological Substrate

提出了一种功能微拓扑结构细胞培养芯片,芯片结构由具有细胞粘附特异性的微平台和微柱阵列交替排布而成,并采用具有良好生物相容性和化学稳定性的聚合物制备,因此该芯片具有对细胞规约能力强和定位稳定性好的优点。芯片性能验证结果表明,所设计的功能微拓扑结构芯片细胞定位率达到90%,芯片结构及尺寸稳定性好,为需要长期细胞培养的干细胞研究提供了重要的支撑工具。

There are two main categories of cell positioning culture chips:chemical modification of surfaces and physical structure methods.The chemical methods have the advantage of providing a strong binding force,but the instability of chemical materials limits its application.The physical methods have the advantage of good stability for cell positioning,while the nonspecificity of physical structure weakens the ability to regulate cells.Here,we propose a functional micro topological chip.The chip is composed of micro-platform and micro-post array with different adhesion characteristics.At the same time,the chip is prepared by a polymer material with good biocompatibility and high chemical stability;therefore,it has the advantages of strong cell constraint ability and good localization stability.The result of chip performance verification shows that the cell localization rate of the functional micro topological chip is up to 90%,and the chip structure and dimension stability are good.The study provides an important supporting tool for the research of stem cells and their differentiation that need long-term cell culture.