共轭高分子PFP/金纳米颗粒合成及用于探测核酸酶的研究

Synthesis of conjugated polymer PFP/gold nanoparticles and its application in detection of nuclease

使用共轭高分子/金纳米颗粒/染料标记的DNA复合物发展了S1核酸酶的一种新型检测方法,此方法利用了金良好的荧光淬灭性质和共轭高分子的信号放大特性。这种方法是由于纳米材料表面能量转移(NSET)中,能量从供体分子到纳米颗粒表面的转移遵循可预测的约为70~100 nm的距离。在此过程中,由于从共轭高分子到嵌入染料进而到金纳米颗粒表面的NSET,不存在S1核酸酶的情况下将观察不到嵌入染料的荧光信号。而存在S1核酸酶的情况下,双链DNA被切离金纳米颗粒的表面,NSET过程中断,从共轭高分子到嵌入染料高效的荧光共振能量转移所得的嵌入染料的荧光得以恢复。

A new detection method of S1 nuclease was developed by using conjugated polymer/gold nanoparticles/dye labeled DNA complex.This method took advantage of the good fluorescence quenching property of gold and the signal amplification property of conjugated polymer.This method was due to the fact that the energy transfer from donor molecules to nanoparticles followed a predictable distance of about 70-100 nm in the nanomaterial surface energy transfer(NSET).In this process,due to the NSET from conjugated polymer to embedded dye,then to gold nanoparticles,the fluorescence signal of embedded dye would not be observed without S1 nuclease.In the presence of S1 nuclease,the double stranded DNA was cut off from the surface of gold nanoparticles,the NSET process was interrupted,and the fluorescence of the embedded dye obtained from the efficient fluorescence resonance energy transfer from the conjugated polymer to theembedded dye was restored.