呋喃妥因代谢物胶体碳免疫试纸条制备与应用

Study of the Colloidal Carbon Immunoassay Strip for the Detection of Furantion Metabolites

以呋喃妥因代谢物AHD作为检测目标物,选择SB4型炭黑作为抗体标记物,使用BB缓冲液分散炭黑颗粒,制得胶体碳溶液并优化胶体碳标记抗体条件。选择Millipore135s膜作为载体膜,将包被原和羊抗兔二抗适当分散后分别喷涂在该膜上,干燥后组装试纸条,建立免疫层析分析方法;确定方法检出限,考查试纸条的灵敏度和稳定性并应用于水产品(鱼类)的检测。最优条件下组装试纸条的方法检出限为1.0μg/L,实际样品选择4种常见鱼类检测,向其中添加AHD标准品,样品经过处理后,使用该试纸条进行检测,检出限为0.2μg/kg。目测即可得出结果,无需借助仪器,检测时间不超过15 min。

In this work,the furantoin metabolite AHD was used as the target and SB4 carbon black as the antibody marker.BB buffer was selected as the dispersion solution of carbon nanoparticles.The millipore 135s membrane is selected as the best NC membrane,the coating antigen and goat anti-rabbit secondary antibody were fixed to nitrocellulose membrance as test line and control line.After drying assembled test strip,the immunochromatographic strip for the determination of salbutamol were deceloped.The detection limit of the method was determined,the sensitivity and stability of the test strip were examined and applied to the detection of aquatic products(FISH).The limit of detection of colloidal carbon immunochromatographic assay for NPAHD is 1.0μg/L.Four kinds of products of animal-derived food were selected as test samples were spiked a certain concentration standards.After derivati-zed and concentrated pretreatment,the samples were directly used for analysis by colloidal ca-rbon immunochromatographic assay.The visual detection limit of the AHD in animal derived food were 0.2μg/kg.The test strip is easy to use,and the results can be obtained by visual inspection.Without the aid of instrument,the test time is not more than 15 minutes.