细菌纤维素/聚己内酯微米纤维复合膜的制备及性能

Preparation and Properties of Bacterial Cellulose/Polycaprolactone Microfibrous Composite Membranes

为制备模拟细胞外基质结构的微纳尺度复合材料,利用静电纺丝技术制备了聚己内酯(Polycaprolactone,PCL)微米纤维膜,通过与纳米尺度的细菌纤维素(Bacterial Cellulose,BC)原位复合,制备了BC/PCL复合纤维支架。采用扫描电镜、红外光谱分析、X射线衍射分析对材料的形貌、结构进行了表征。通过单轴力学测试对复合材料力学性能进行了研究,并利用成纤维细胞对复合材料的生物相容性进行评价。结果表明:通过静电纺丝法制备的PCL微米纤维的平均直径,随聚合物纺丝液质量分数的增加有增加的趋势,BC与PCL微米纤维复合后,BC纳米纤维渗透入微米纤维膜内部,实现微纳米纤维较好的复合。红外光谱分析和X射线衍射分析进一步证明BC和PCL微米纤维成功复合。PCL微米纤维膜复合BC膜后,相比PCL微米纤维膜增加了其断裂强度,同时复合支架无明显细胞毒性,可应用于生物医学领域。

In order to prepare micro/nanoscale composites to mimic the structures of extracellular matrices,polycaprolactone(PCL)microfibrous membranes were prepared with electrospinning technology,and bacterial cellulose/polycaprolactone(BC/PCL)composite fibrous scaffolds were prepared by in situ compounding with nanoscale BC.The morphology and structure of the material were characterized by scanning electron microscopy,infrared spectroscopy and X-ray diffraction analysis.The mechanical properties of the composites were studied via uniaxial mechanical test,and the biocompatibility of the composites was evaluated via fibroblasts.The results showed that the average diameter of PCL microfibers prepared by electrospinning tends to increase with the increase of the mass fraction of polymer spinning solution.As to the combination of bacterial cellulose and PCL microfibers,BC nanofibers infiltrate into microfibers membrane to achieve composite of microfibers and nanofibers.Infrared spectrum analysis and X-ray diffraction analysis have further proved the success in the combination of BC and PCL microfibers.The breaking strength of composite membrane prepared by compounding PCL microfibers with BC membranes is higher in comparison with PCL microfibrous membrane.Meanwhile,the composite scaffolds don t exhibit obvious cytotoxicity,so they can be applied in biomedical field.