反相高效液相色谱法测定荠菜中的维生素C

Determination of Vitamin C in Capsellae by RP-HPLC

本试验以超声波辅助提取荠菜中VC,并采用反相高效液相色谱法测定其含量。先将荠菜匀浆后,以2%草酸为萃取溶剂,100 W的功率,25℃超声提取10 min,0.45μm微孔滤膜过滤,得到VC提取液,再进行反相高效液相色谱测定,以0.1%草酸溶液为流动相,流速1.0 mL/min,采用C18反相色谱柱分离,在246 nm波长定量检测。结果表明,超声提取荠菜中VC含量为56.05 g/100 g;VC浓度在2~20μg/mL范围内峰面积与VC浓度间线性良好,相关系数为0.9998;VC含量的检出限为0.6 mg/100 g,定量限为2 mg/100 g。方法回收率为99.73%~101.48%;重复性的相对标准偏差为1.97%。可见该方法能有效提取蔬菜中VC,测定结果稳定,重现性好,可以用于蔬菜中VC的提取与检测。

In this experiment,VC in capsellae was extracted by ultrasonic assisted method,and its content was determined by RP-HPLC.Capsella after homogenate was extracted by ultrasonic assisted method with 2%oxalic acid as the extraction solvent,100 W power,25℃for 10 min.Then the samples were filtered through 0.45μm membrane,detected by RP-HPLC with Hypersil C18 column.Mobile phase was 0.1%oxalic acid with flow rate 1.0 mL/min,ultraviolet detection wavelength was at 246 nm.The results showed that the content of VC in capsella was 56.05 g/100 g.When the concentration of vitamin C were 2-20μg/mL,the linear relation between peak area and concentration of vitamin C were good.And the coefficient of correlation for the regression equation was 0.9998.The detection limits was 0.6 mg/100 g.The detection limits of quantification was 2 mg/100 g.The recovery for the vitamin C was in the range from 99.73%to 101.48%,with the relative standard deviation(RSD)was 1.97%.This method had good precision and high accuracy.This method can determine vitamin C in vegetables.