干扰素α和胸腺五肽协同干预对HepG2.2.15细胞APOBEC3A和APOBEC3B mRNA表达的影响

Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells

目的探讨IFNα和胸腺五肽(TP5)协同干预对HepG2.2.15细胞载脂蛋白B mRNA编辑酶催化多肽3A(APOBEC3A)、APOBEC3B基因mRNA表达水平的影响。方法将HepG2.2.15细胞分为空白对照组、IFN处理组、TP5处理组和IFNα联合TP5组,在处理的12、24、48和72 h用实时荧光定量PCR方法检测细胞APOBEC3A、APOBEC3B基因mRNA表达水平。计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验。结果与空白对照组相比,IFNα、IFNα联合TP5分别处理12、24、48和72 h后,APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义(P值均<0.001)。与IFNα处理组相比,4个时间点IFNα联合TP5组APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义(P值<0.001)。TP5处理在各时间点对APOBEC3A mRNA表达水平的影响差异无统计学意义(P值均>0.05)。与对照组相比,所有处理组中APOBEC3B的mRNA表达水平差异无统计学意义(P值均>0.05)。结论IFNα联合TP5处理可显著上调HepG2.2.15细胞中APOBEC3A mRNA的表达水平。

Objective To investigate the effect of synergistic intervention of interferonα(IFNα)and thymopentin(TP5)on the mRNA expression of apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 A(APOBEC3 A)and apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 B(APOBEC3 B)in HepG2.2.15 cells.Methods HepG2.2.15 cells were divided into blank control group,IFNαtreatment group,TP5 treatment group,and IFNα+TP5 treatment group,and at 12,24,48,and 72 hours of treatment,quantitative real-time PCR was used to measure the mRNA expression of APOBEC3 A and APOBEC3 B in HepG2.2.15 cells.An analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups.Results Compared with the blank control group,the IFNαtreatment group and the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at 12,24,48,and 72 hours of treatment(all P<0.001).Compared with the IFNαtreatment group,the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at these four time points(all P<0.001).TP5 treatment had no significant influence on the mRNA expression of APOBEC3 A at each time point(all P>0.05).There was no significant difference in the mRNA expression of APOBEC3 B between the blank control group and the treatment groups(all P>0.05).Conclusion IFNαcombined with TP5 can significantly upregulate the mRNA expression of APOBEC3 A in HepG2.2.15 cells.