27个Y-STR基因座在中国3个民族的遗传多态性及与其他13个群体的聚类分析

Genetic polymorphisms of 27 Y-STR loci in 3 Chinese population and the cluster analysis of 13 ethnic groups

目的研究河南回族、贵州仡佬族和贵州苗族人群的27个Y-STR基因座的遗传多态性及与其他13个群体间的遗传关系,探讨其在法医学和群体遗传学研究中的应用价值。方法采用STRtyper-27Y复合扩增体系分别对河南回族(1080例)、贵州仡佬族(297例)、贵州苗族(221例)无血缘关系的男性个体的27个Y-STR基因座进行扩增,应用3500XL遗传分析仪进行基因分型,采用Mega6.0软件进行群体间的AMOVA分析、聚类分析。结果河南回族、贵州仡佬族和贵州苗族人群的Y-STR基因座单倍型多样性分别为0.9999725、0.9997271和0.9998355,系统鉴别能力分别为0.9851852、0.9629630和0.9819004。AMOVA分析显示,16个人群间的遗传距离在-0.0001~0.2120,相同民族间遗传距离较低,而不同民族群体间遗传距离普遍较大。结论27个Y-STR基因座在河南回族、贵州仡佬族和贵州苗族人群中具有丰富的遗传多态性,研究数据对建立Y染色体数据库、群体遗传学研究和法医学应用中具有重要的参考价值。

Objective To investigate the genetic polymorphisms of 27 Y-chromosomal short tandem repeats(Y-STR)loci in 3 ethnic populations of China,explore the population genetic relationship with 13 neighboring populations and evaluate their values on forensic and population genetics.Methods A total of 27 Y-STR loci of male individuals in the 1080 Huis of Henan,297 Gelaos of Guizhou and 221 Miaos of Guizhou were amplified with STRtyper-27Y Kit,and the genotyping were detected with 3500XL Genetic Analyzer.Mega6.0 software were used for AMOVA analysis and clustering analysis.Results In the Huis of Henan,Gelaos of Guizhou and Miaos of Guizhou,the haplotype diversity(HD)of Y-STR loci were 0.9999725,0.9997271 and 0.9998355,respectively,and the discrimination capacity(DC)were 0.9851852,0.9629630 and 0.9819004,respectively.AMOVA analysis showed that the genetic distance among 16 populations ranged from-0.0001 to 0.2120,the genetic distance between the same ethnic groups was lower,while the genetic distance between different ethnic groups was generally larger.Conclusion Twenty-seven Y-STR loci have abundant genetic polymorphisms in the Huis of Henan,Gelaos of Guizhou and Miaos of Guizhou,which are of important significance for establishing a Y-STR database,studying population genetics and in forensic practice.