运用协同戊巴比妥钠动物模型初步探究闽产三味中药的镇静催眠活性

The Sedative and Hypnotic Activities of Three Traditional Chinese Medicines Produced in Fujian Province were Preliminarily Studied by Using the Animal Model of Synergistic Pentobarbital Sodium

目的运用协同戊巴比妥钠动物模型实验初步探讨闽产莲子、灵芝和养心草三味中药水提液的镇静催眠活性,为后期探讨其镇静催眠活性机制提供科学实验依据。方法以成年人临床拟用剂量作为受试药物拟用量,高、中、低剂量组分别等于拟用剂量的2倍、1倍、0.5倍;以艾司唑仑为阳性对照组,蒸馏水为空白对照组。随机分配小鼠,每组10只。协同戊巴比妥钠阈下剂量试验:持续灌胃7天,末次给药30min后,给予小鼠腹腔注射阈下剂量戊巴比妥钠,记录入睡小鼠只数,统计入睡率。协同戊巴比妥钠阈剂量试验:按上法给药,末次给药30min后给予小鼠腹腔灌注阈剂量的戊巴比妥钠,统计小鼠的睡眠持续时间。结果莲子高剂量,灵芝高剂量组,养心草中、高剂量组均能明显增加小鼠睡眠只数(P<0.05),并且能够显著提高小鼠的入睡百分率(P<0.05)。养心草中、高剂量组可显著增加小鼠睡眠时长(P<0.05)。结论莲子具有安神活性;灵芝具有镇静安神活性;养心草有镇静催眠活性。

OBJECTIVE The sedative and hypnotic activity of water extracts of lotus seed,Ganoderma lucidum and Sedum aizoon L.,which were produced in Fujian province were preliminarily studied by synergistic pentobarbital sodium animal model experiment.It provides a basis for exploring the mechanism of their sedative and hypnotic activity.METHODS Taking the clinically used dose of the adult as the dose of the test drug,and the high,medium and low dose groups were equal to 2 times,1 times and 0.5 times of the intended doses respectively.Estazolam was used in the positive control group and distilled water was in blank control group.Ten mice were randomly assigned to each groups.Synergistic subthreshold dose test of pentobarbital sodium:Mice were given gavage for 7 days.They were given intraperitoneal injection of subthreshold dose of pentobarbital sodium 30 minutes after the last intragastric administration,and the number of sleeping mice were recorded and the sleep rate was calculated.Synergistic pentobarbital sodium threshold dose test:the same as the above method,mice were given intraperitoneal injection of pentobarbital sodium threshold dose 30 minutes after the last dose,and the sleep duration was recorded.RESULTS The number of mice in the high-dose group of lotus seed,Ganoderma lucidum,the medium and high-dose group of Sedum aizoon L.were significantly increased(P<0.05),and the percentage of sleep was significantly increased(P<0.05).The medium and high dose of Sedum aizoon L.significantly increased the sleep duration of mice(P<0.05).CONCLUSION Lotus seeds have a certain calming effect;Ganoderma lucidum has a certain sedative and tranquilizing effect;Sedum aizoon L.has a good sedative and hypnotic effect.