黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化的影响

Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line

目的探究黏着斑激酶抑制剂TAE226对人口腔鳞状细胞癌细胞上皮间质转化(EMT)过程的影响。方法不同浓度(0、1、5、10μmol·L-1)的TAE226作用于人口腔鳞状细胞癌HSC-3和HSC-4细胞24、48、72 h后,实时定量聚合酶链反应检测EMT标志物E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)的mRNA表达;蛋白质印迹法检测E-cadherin、Vimentin在TAE226作用48 h后的蛋白表达。结果实时定量聚合酶链反应检测表明,随着TAE226作用时间和浓度的增加,E-cadherin mRNA的表达增加,Vimentin mRNA的表达降低(P<0.05)。蛋白质印迹法检测表明,随着TAE226浓度的增加,E-cadherin蛋白的表达增加,Vimentin蛋白的表达降低(P<0.05)。结论TAE226能有效抑制人口腔鳞状细胞癌细胞株的EMT进程,有望成为治疗口腔鳞状细胞癌的有效药物之一。

Objective To study the effect of the focal adhesion kinase inhibitor TAE226 on epithelial-mesenchymal transition(EMT)in human oral squamous cell carcinoma(OSCC)cell line.Methods HSC-3 and HSC-4 cells were cultured with TAE226 under different concentrations(0,1,5,and 10μmol·L-1)for 24,48,and 72 h.Real-time quantitative polymerase chain reaction was performed to detect the mRNA expressions of E-cadherin and Vimentin.The protein expressions of E-cadherin and Vimentin were determined by Western blot assay after 48 h of TAE226 treatment.Results Real-time quantitative polymerase chain reaction showed that increasing the TAE226 dose and reaction time resulted in increased and decreased E-cadherin and Vimentin mRNA expressions,respectively(P<0.05).Western blot assays showed that increasing the TAE226 dose resulted in increased and decreased E-cadherin and Vimentin protein expressions,respectively(P<0.05).Conclusion TAE226,which is expected to be an effective drug for OSCC treatment,can effectively inhibit the EMT of the OSCC cell line.