HPLC同时测定鸢都感冒颗粒中新绿原酸、绿原酸、葛根素和大豆苷元的含量

Simultaneous Determination of Neochlorogenic Acid,Chlorogenic Acid,Puerarin and Daidzein in Yuandu Ganmao Granules by HPLC

目的建立测定鸢都感冒颗粒中新绿原酸、绿原酸、葛根素和大豆苷元含量的方法。方法采用HPLC,色谱柱为Agilent XDB-C18(250 mm×4.6 mm,5μm);柱温40℃;流动相为乙腈-0.1%磷酸,梯度洗脱;在327 nm波长处测定新绿原酸和绿原酸,在250 nm波长处测定葛根素和大豆苷元。结果新绿原酸在0.0019~0.1173μg范围内,葛根素在0.0066~4.0038μg范围内,绿原酸在0.0039~0.2378μg范围内,大豆苷元在0.0017~0.0696μg范围内线性关系良好;平均加样回收率分别为99.5%,99.0%,99.4%,99.9%,RSD分别为0.9%,1.8%,1.8%,1.8%。结论此方法准确性和灵敏度较高,为鸢都感冒颗粒的含量测定提供了科学依据。

Objective To build a new method for the simultaneous determination of neochlorogenic acid,chlorogenic acid,puerarin and daidzein in Yuandu Ganmao Granules.Methods HPLC was adopted.The separation was performed on an Agilent ZORBAX XDB-C18(250 mm×4.6 mm,5μm)with temperature of 40℃.Gradient elution was performed,using acetonitrile-0.1% phosphoric acid as mobile phase.The detection wavelength was set at 327 nm for neochlorogenic acid and chlorogenic acid,and 250 nm for puerarin and daidzein.Results Good linearity was achieved in the range of 0.0019-0.1173μg,0.0066-4.0038μg,0.0039-0.2378μg and 0.0017-0.0696μg for neochlorogenic acid,puerarin,chlorogenic acid and daidzein,respectively.The average recoveries of the above components were 99.5%,99.0%,99.4%and 99.9%,with RSDs of 0.9%,1.8%,1.8%and 1.8%,respectively.Conclusion This method is accurate and sensitive,and can be used for determination of Yuandu Ganmao Granules.