黄连解毒散和黄霉素对不同日龄肉鸡回肠菌群结构的影响

Effects of Coptidis Powder for Detoxification and Flavomycin on Ileal Microflora in Broilers of Different Ages

为了研究饲料中添加黄连解毒散(CPD)和黄霉素(FLA)对肉鸡回肠肠道菌群结构的影响,选取180只1日龄爱拔益加(AA)肉仔鸡,随机分成3组,分别饲喂基础饲粮(对照组)、基础日粮添加5 mg/kg FLA(FLA组)、基础日粮添加1.25 g/kg CPD(CPD组)。结果表明,随着日龄的增加,各组肉鸡的回肠微生物多样性逐渐下降,其中FLA组和CPD组35日龄时操作分类单元(OTU)丰度显著低于15和25日龄(P<0.05)。与对照组相比,日粮中添加FLA显著降低了35日龄肉鸡回肠菌群中黄杆菌属、链球菌属和拟无枝酸菌属等10个属的相对丰度(P<0.05);添加CPD显著降低了35日龄肉鸡回肠菌群中克雷伯菌属、黄杆菌属和拟无枝酸菌属等6个属的相对丰度(P<0.05)。不同处理组肉鸡回肠微生物群落在代谢途径和蛋白质功能上也存在显著差异。35日龄时,与FLA组相比,CPD组肉鸡回肠菌群信号分子与相互作用功能显著增加(P<0.05),同时转录及细胞支架功能显著降低(P<0.05)。由此可见,肉鸡日粮中添加CPD可以调节回肠菌群结构,与FLA作用相似,且不同微生物群落中功能基因的代谢途径也随日粮而变化。

In order to study the effect of coptidis antidotal powder(CPD)and flavomycin(FLA)on the structure of intestinal microflora in the ileum of broilers,one hundred and eighty one-day-old AA broilers were randomly divided into three groups:basal diet(CK),basal diet with 5 mg/kg FLA(FLA group),basal diet with 1.25 g/kg CPD(CPD group).The results showed that the diversity of ileal microflora decreased with the increase of age,and the abundance of operational taxonomic unit(OTU)in FLA and CPD groups at 35 days of age was significantly lower than that at 15 and 25 days of age(P<0.05).Compared with the control group,dietary FLA significantly decreased the relative content of 10 genera in ileal flora of 35-day-old broilers,such as Flavobacterium,Streptococcus and Amycolatopsis(P<0.05),and CPD significantly decreased the relative content of 6 genera in ileal flora of 35-day-old broilers,such as Klebsiella,Flavobacterium and Amycolatopsis(P<0.05).There were also significant differences in the metabolic pathway and protein function of ileum microbial community among different treatment groups.At 35 days of age,compared with FLA group,the signalling molecules and interaction function of ileal flora in CPD group increased significantly(P<0.05),while transcription and cytoskeleton decreased significantly(P<0.05).It could be seen that CPD supplement in broiler diet could regulate the structure of ileum microbiota,similar to FLA.The metabolic pathways of the functional genes in different microbial communities were found to vary with diet.