Photoshop在微球直径测量与药材显微结构绘图上的运用探索

Application of Photoshop in Measurement of Microsphere Diameter and Microstructure Drawing of Medicinal Materials

目的:探究Photoshop图像处理软件在药剂学的微球直径测量和鉴定学的药材组织结构绘图的可行性,为中药学的微观结构测量和绘图提供一种新方法。方法:应用Photoshop(CS3版本以上)软件对同批次的200个三七总皂苷白蛋白微球直径进行测量;应用数码显微摄像和Photoshop图像处理软件绘制三七(NotoginsengRadix et Rhizoma)药材粉末及其4种常见伪品白及(Bletillae Radix),高良姜(Alpiniae Officinarum Rhizoma),姜黄(Curcumae Longae Rhizoma),木薯(Manihot esculenta)粉末的显微结构示意图并制作鉴别检索表对其进行分类鉴别。结果:该批次微球直径均值为(30. 62±4. 21)μm,直径主要分布在20~40μm,经激光粒度分析仪验证微球直径均值为(30. 18±4. 67)μm,2种方法比较差异无统计学意义;三七粉末中淀粉粒众多,无草酸钙针晶、油细胞、棕色素、石细胞,可以通过显微鉴别三七粉末与4种常见伪品。结论:Photoshop图像处理软件测量微球直径速度快、准确度高、简便、对测量仪器要求不高,为快速测量微球直径提供了一条新途径。Photoshop图像处理软件绘制显微结构示意图方便快捷、更好地反映显微组织结构的原始形态,为显微结构的绘制提供了一种新方法。

Objective: To explore the feasibility of Photoshop image processing software in the microspherical diameter measurement and identification of medicinal materials,in order to provide a new method for the measurement and mapping of traditional Chinese medicine. Method: Photoshop( CS3 version and above) software was used to measure the diameter of 200 Notoginseng Radix et Rhizoma albumin microspheres in the same batch,digital microscopy and Photoshop image processing software was used to draw schematic diagrams of microstructures of Notoginseng Radix et Rhizoma medicinal powders and its four common pseudo-products and powders,namely Bletillae Radix,Alpiniae Officinarum Rhizoma,Curcumae Longae Rhizoma and Manihot esculenta,and make the identification table for classification and identification. Result: The average diameter of the microspheres was( 30. 62 ± 4. 21) μm,and the diameter was mainly distributed in 20-40 μm. The average diameter of the microspheres was verified by laser particle size analyzer( 30. 18 ± 4. 67) μm. The differences between the two methods were not statistically significant. There were many starch granules in Notoginseng Radix et Rhizoma powder,with no calcium oxalate needle,oil cell,brown pigment and stone cell. Microscopic identification could be made for Notoginseng Radix et Rhizoma powder and 4 common counterfeits. Conclusion: Photoshop image processing software measures the diameter of the microspheres with a high speed,high accuracy,simplicity,and low requirements for measuring instruments. It provides a new way to quickly measure the diameter of microspheres. Photoshop image processing software draws a schematic diagram of the microstructure, which is convenient and faster. The original morphology of the ground-reactive microstructure provides a new method for the microstructural drawing.