组蛋白赖氨酸去甲基化酶4C对卵巢癌SKOV3细胞恶性生物学行为的影响

Effect of Recombinant Lysine Specific Demethylase 4C on malignant biological behavior of SKOV3 cells in ovarian cancer

目的分析组蛋白赖氨酸去甲基化酶4C(recombinant lysine specific demethylase 4,KDM4C)对卵巢癌SKOV3细胞恶性生物学行为的调控作用。方法建立KDM4C下调的稳定SKOV3细胞系;采用CCK8、平板克隆实验、划痕实验及Transwell方法检测KDM4C对卵巢癌SKOV3细胞体外恶性生物学行为的影响;采用裸鼠皮下荷瘤实验,检测KDM4C对卵巢癌SKOV3细胞,体内增殖成瘤能力的影响;采用t检验分析各组间差异。结果在卵巢癌SKOV3细胞系中,对KDM4C进行下调时,其体外迁移、侵袭、增殖及成瘤方面恶性生物学行为显著减弱(P<0.05),其体内增殖及成瘤能力显著减弱(P<0.05)。结论KDM4C高表达能显著促进卵巢癌细胞的恶性生物学行为,抑制KDM4C可能是卵巢癌靶向治疗的潜在靶点之一。

Objective To analyze the regulatory effect of Recombinant Lysine Specific Demethylase 4C(KDM4C)on malignant biological behavior of SKOV3 cells in ovarian cancer.Methods Stable SKOV3 cell lines down⁃regulated by KDM4C were established.The effects of KDM4C on malignant biological behavior of ovarian cancer SKOV3 cells in vitro were detected by CCK8 assay,plate cloning assay,scratch assay and Transwell method.The effect of KDM4C on SKOV3 cell proliferation and tumorigenic ability of ovarian cancer was detected by subcutaneous tumor⁃bearing experiment in nude mice.T test was used to analyze the differences between groups.Results In SKOV3 cell lines of ovarian cancer,when KDM4C was down⁃regulated,its malignant biological behavior in vitro migration,invasion,proliferation and tumorigenesis were significantly reduced(P<0.05),and its ability of proliferation and tumorigenesis in vivo was significantly reduced(P<0.05).Conclusion High expres⁃sion of KDM4C can significantly promote the malignant biological behavior of ovarian cancer cells,and inhibiting KDM4C may be one of the potential targets of targeted therapy for ovarian cancer.