脓毒症患者血清IL-3的表达情况及其临床意义

Expression of interleukin-3 in patients with sepsis and its clinical significance

目的:检测不同程度脓毒症患者血清白介素-3(interleukin-3,IL-3)的表达情况,并探究其与疾病的相关性。方法:纳入2017年12月至2018年12月符合条件的脓毒症病例组100例,分为非休克组(n=69)和休克组(n=31),存活组(n=79)和死亡组(n=21)。并纳入同时期健康体检人群50例作为对照组。收集患者的血尿样本检测包括IL-3等相关生化指标,同时记录患者急性病生理学和长期健康评价Ⅱ(acute physiology and chronic health evaluation,APACHEⅡ)评分以及序贯器官衰竭评分(sequential organ failure assessment,SOFA),作为评估疾病严重程度的指标。结果:脓毒症病例组血清IL-3水平显著高于健康对照组(P<0.01),休克组血清IL-3水平高于非休克组(P<0.01),死亡组血清IL-3水平高于存活组(P<0.01);脓毒症患者血清IL-3水平与APACHEⅡ评分(r=0.67,P=0.02)及SOFA(r=0.59,P=0.03)呈正相关联系;脓毒症患者血清IL-3水平是患者发生死亡事件的独立危险因素(P=0.03);IL-3水平判断脓毒症死亡预后的ROC曲线下面积为0.88,灵敏度为0.86,特异度为0.72(P=0.03)。结论:脓毒症患者血清IL-3水平升高,且与疾病严重程度具有相关性,可用于评估脓毒症患者预后。

Objective:To detect the serum levels of interleukin-3(IL-3)in patients with sepsis and its clinical significance.Methods:One hundred cases of sepsis in our hospital from December 2017 to December 2018 were divided into a non-shock group(n=69),a shock group(n=31),a survival group(n=79),and death group(n=21).Fifty healthy people in the same period were enrolled as the control group.Blood and urine samples were collected for the measurement of IL-3 and other related biochemical indicators.Acute physiology and chronic health evaluation II(APACHE II)and sequential organ failure assessment(SOFA)were recorded as indicators to assess the severity of the disease.Results:Serum levels of IL-3 in sepsis group were significantly higher than those in healthy control group(P<0.01),while serum levels of IL-3 in shock group were higher than those in non-shock group(P<0.01),and serum levels of IL-3 in death group were higher than those in survival group(P<0.01).Serum levels of IL-3 was positively correlated with APACHE II score(r=0.67,P=0.02)and SOFA score(r=0.59,P=0.03).Serum levels of IL-3 was an independent risk factor for death events in sepsis patients(P=0.03);the ROC curve area under which IL-3 level judged the prognosis of sepsis death was 0.88,sensitivity was 0.86,specificity was 0.72(P=0.03).Conclusion:Serum levels of IL-3 in sepsis patients are elevated and correlated with the severity of the disease.It can be used to evaluate the prognosis of sepsis.