基于生物信息学miR-221调控前列腺癌分子机制的研究

Study on the Molecular Mechanism of Prostate Cancer Regulated by Bioinformatics miR-221

目的:基于GEO数据库对miR-221调控前列腺癌差异基因进行分析,以期找到miR-221调控前列腺癌的重要靶点基因。方法:在GEO数据库里,获得miR-221调控前列腺癌差异基因表达的芯片GSE45627,用在线分析工具GEO2R进行数据分析,按照P<0.01和差异倍数≥2进行筛选,将筛选得到的基因输入DAVID数据库进行基因和通路的富集分析,然后进行蛋白质互做分析。结果:共鉴定108个DEG。基因本体富集结果表明,DEG编码的功能分子主要位于细胞质、细胞核,分子功能主要涉及“双链RNA结合”“螺旋酶活性”“双链DNA结合”“蛋白结合”“单链RNA结合”,与生物学过程有关,包括“调节Ⅰ型干扰素信号通路”“病毒的防御反应”“调控病毒遗传物质的复制”“先天免疫反应”。KEGG信号通路分析主要涉及甲型H1N1流感信号通路、麻疹信号通路、丙型肝炎信号通路、RIG-I样受体信号通路、Toll样受体信号通路等。结论:生物信息分析的结果可以发现miR-221调控前列腺癌中有用的靶点和信号通路,但这些需要进一步的实验验证。

Objective:To analyze the differential genes regulated by miR-221 in prostate cancer based on GEO database in order to find an important target gene for miR-221 regulation of prostate cancer.Methods:In GEO database,the chip GSE45627,which was obtained by miR-221 regulating differential gene expression in prostate cancer,was analyzed by online analysis tool GEO2R.According to P<0.01and difference multiple≥2,the obtained genes were input into DAVID database for gene and pathway enrichment analysis,and then protein interaction analysis and module analysis were carried out.The analysis results are compared with those of text mining.Results:A total of 108 DEG were identified.The results of gene ontology enrichment showed that the functional molecules encoded by DEG were mainly located in cytoplasm,nucleus,and their molecular functions were mainly related to“double-stranded RNA binding”“spiroenzyme activity”“double-stranded DNA binding”“protein binding”and“single-stranded RNA binding”,which were related to biological processes,including“regulating typeⅠinterferon signaling pathway”“defensive response to virus”and“negative regulation of viral genome replication”.KEGG signal pathway analysis mainly involves A H1N1 influenza signal pathway,measles signal pathway,hepatitis C signal pathway,RIG-I like receptor signal pathway,Toll-like receptor signal pathway and so on.Conclusion:The results of bioinformation analysis can find useful targets and signaling pathways in the regulation of prostate cancer by miR-221,but these need to be further verified by experiments.