结肠癌组织中Raf激酶抑制蛋白的表达及意义

Expression and significance of raf kinase inhibitor protein in colon carcinoma tissue

目的检测人结肠癌组织中Raf激酶抑制蛋白(RKIP)的表达。方法采用免疫组织化学SP法检测60例结肠癌组织和60名正常结肠黏膜组织中的RKIP蛋白的表达。结果正常结肠黏膜组织中RKIP蛋白的阳性表达率为88%(53/60),高于结肠癌组织的48%(29/60)(P<0.05)。结肠癌组织中RKIP蛋白的表达与肿瘤的分化程度、浸润深度和淋巴结转移有关(P<0.05),与肿瘤患者性别、年龄、肿瘤生长部位、生长方式及有无脉管侵犯无关(P>0.05)。结论RKIP的低表达可能与结肠癌的发生和肿瘤细胞的浸润转移能力相关;检测RKIP的表达对判断结肠癌预后和转移有重要参考价值。

Objective To investigate the expression of raf kinase inhibitor protein(RKIP)in colon carcinoma tissue.Methods The expressions of RKIP in 60 cases of colon carcinoma tissue and normal colon mucosa tissues were detected by using immunohistochemistry(SP)methods.Results The express of RKIP in normal colon mucosa tissue was significantly higher than that in carcinoma tissue(P<0.05).The expression of RKIP in colon carcinoma tissue was signi-ficantly associated with differentiation,invading grade and lymph nod metastasis of colon carcinoma(P<0.05).On the contrary,it was not correlated with the gender,age,or the site of tumor growth,growth pattern and venous invasion(P>0.05).Conclusion The low level expression of RKIP may play an important role in occurrence of colon carcinoma and the ability of the invasion,metastasis and in predicting the carcinoma of rectum prognosis and metastasis.