ROR1在卵巢上皮性肿瘤中的表达及其与临床预后的关系

Expression of ROR1 in ovarian epithelial tumors and its relationship with clinical prognosis

目的:分析受体酪氨酸激酶样孤儿受体-1(the receptor tyrosine kinase-like orphan receptors-1, ROR1)在卵巢上皮性肿瘤中的表达,并探讨其表达对卵巢癌患者临床预后的意义。方法:选取南京医科大学附属妇产医院2013年1月—2018年12月行根治性手术的卵巢癌患者的组织标本55例,并随机选取同期卵巢交界性肿瘤和良性肿瘤各30例,以及非卵巢病变进行切除的正常卵巢15例为对照组,免疫组织化学检测各组ROR1的表达情况。除6例外,所有卵巢癌患者均随访,末次随访时间为2019年3月30日,分析ROR1与上皮性卵巢癌各项临床病理参数的相关性,生存分析采用KaplanMeier法。结果:ROR1在卵巢癌中的表达高于正常卵巢组织及卵巢交界性和良性肿瘤,卵巢癌的ROR1表达在不同肿瘤的FIGO分期及有无淋巴结转移间差异有统计学意义(P<0.05),而与患者年龄、组织学分型及分化程度之间无明显相关性,Kaplan-Meier生存分析显示卵巢癌ROR1阳性表达组与阴性表达组生存时间差异有统计学意义(P<0.05)。结论:卵巢癌组织中ROR1蛋白表达升高,可能作为评估患者预后的潜在指标,对卵巢癌的诊治提供一定的理论依据。

Objective:To investigate the expression of the receptor-tyrosine-kinase-like orphan receptor-1(ROR1)in epithelial ovarian tumors,and to analyze the clinicopathologic significance in the patients with ovarian cancer.Methods:55 cases of ovarian cancer patients were selected in Women’s Hospitol of Nanjing Medical University between January 2013 and December 2018.In the same period,30 cases of borderline tumor and benign tumor were selected random as control groups,also included 15 cases of normal ovarian.The expression of ROR1 protein in epithelial ovarian cancer and control tissues were detected by immunohistochemistry.All but six of the ovarian cancer patients were followed up.Last follow-up date was March 30,2019.The correlation between ROR1 and various clinicopathological parameters of epithelial ovarian cancer was analyzed.Kaplan-Meier method was used for survival analysis.Results:The expression of ROR1 in ovarian cancer is higher than that in normal ovarian tissue and ovarian borderline and benign tumors.High ROR1 expression was associated with FIGO stage and positive lymph node metastasis.There was no significant correlation with the patient’s age,histologic type and tumor grade.Kaplan-Meier survival analysis showed that the survival time is different between the group of ROR1 positive expression and the group of ROR1 negative expression,the above differences are statistically significant(P<0.05).Conclusion:The expression of ROR1 protein in ovarian cancer tissues is elevated and it can be used as a potential indicator to evaluate the prognosis of patients,and it may provide certain theoretical basis for the diagnosis and treatment of ovarian cancer.