摘要
Au-Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-7(MMP-7)in vitro.The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate(FITC)and 5-Carboxytetramethylrhodamine(5-TAMRA)dyes are attached to the surface of Au nanoparticles(NPs).The nanoprobe can be specifically cut off by MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers.The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7,but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding"false positive"results.This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.
Au–Se bond strategy has been applied to construct fluorescence nanoprobe that is being used to simultaneously dual-image the tumor markers matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-7(MMP-7) in vitro. The two Se-terminating ligand peptide chains respectively labeled with fluorescein isothiocyanate(FITC) and 5-Carboxytetramethylrhodamine(5-TAMRA) dyes are attached to the surface of Au nanoparticles(NPs). The nanoprobe can be specifically cut off by MMP-2 and MMP-7 respectively to carry out significant enhanced fluorescence recovery for simultaneously imaging both markers. The nanoprobe not only displays high selectivity and sensitivity towards MMP-2 and MMP-7, but also has strong anti-interference stability against biothiols and enhanced fidelity for avoiding "false positive" results. This approach offers new perspectives in designing tumor-related early diagnosis approaches and also provides strategies for clinical applications.
基金
supported by the National Natural Science Foundation of China(21535004,91753111,21575081,21775091,21705098)
the Key Research and Development Program of Shandong Province(2018YFJH0502)
the Natural Science Foundation of Shandong Province(ZR201702200428)
the Project of Shandong Province Higher Educational Science and Technology Program(J17KA116)
