Delta/Notch样表皮生长因子相关受体在三阴性乳腺癌中的表达及临床意义

Expression of Delta/Notch-like epidermal growth factor-related receptor in triple negative breast cancer and its clinical significance

目的探讨Delta/Notch样表皮生长因子相关受体(DNER)在三阴性乳腺癌(TNBC)中的表达情况,并分析其与患者的临床病理特征之间的关系。方法收集2017年3月至2018年3月川北医学院附属医院收治的39例TNBC患者的癌组织及癌旁正常组织标本进行回顾性研究,运用组织切片免疫组织化学法检测DNER蛋白表达水平,用RT-PCR检测组织中DNER mRNA表达水平。用配对χ^2检验(McNemar检验)比较乳腺癌组织及癌旁正常组织中DNER阳性率的差异,用χ^2检验或Fisher精确概率法分析DNER蛋白表达与患者临床病理特征之间的关系。用配对t检验比较DNER mRNA在乳腺癌组织与癌旁正常组织中的表达水平,用方差分析和独立样本t检验分析DNER mRNA表达与乳腺癌患者临床病理特征的关系,两两比较用LSD法。结果DNER蛋白在TNBC组织中的阳性表达率为82.1%(32/39),高于在癌旁正常组织中的阳性表达率(23.1%,9/39)(P<0.001)。DNER蛋白在TNBC组织中的阳性表达率与肿瘤直径(χ^2=6.121,P=0.026)、淋巴结转移(P=0.005)及TNM分期(P=0.030)有关。RT-PCR检验结果显示,DNER mRNA在TNBC组织中的相对表达量高于其癌旁正常组织(0.768±0.067比0.487±0.041,t=4.793,P<0.001);DNER mRNA在TNBC组织中的表达与肿瘤直径、TNM分期及淋巴结转移有关(F=8.392,P=0.001;t=5.178,P<0.001;t=4.925,P<0.001)。结论DNER蛋白及mRNA的高表达与TNBC的发生、发展及转移密切相关,可能是乳腺癌诊断和治疗的潜在靶标。

Objective To explore the expression of Delta/Notch-like epidermal growth factor-related receptor(DNER)in triple negative breast cancer(TNBC)and analyze its correlation with clinicopathological characteristics of TNBC patients.Methods The samples of cancer tissues and adjacent normal tissues were collected from 39 cases of TNBC in the Affiliated Hospital of North Sichuan Medical College from March 2017 to March 2018 for a retrospective study.The protein and mRNA expressions of DNER in samples were detected by immunohistochemistry and RT-PCR,respectively.The paired χ^2 test(McNemar test)was used to compare the DNER-positive rates between breast cancer tissues and adjacent normal tissues.χ^2 test or Fisher’s exact test was used to analyze the relationship between DNER protein expression and clinicopathological characteristics of TNBC patients.The DNER mRNA expression was compared between breast cancer tissues and adjacent normal tissues by paired t test.The correlation between DNER mRNA expression and clinicopathological characteristics was analyzed by independent-sample t test and analysis of variance.Pairwise comparison was performed using the LSD test.Results Immunohistochemical results showed that the positive rate of DNER protein in TNBC tissues was 82.1%(32/39),significantly higher than 23.1%(9/39)in adjacent normal tissues(P<0.001),and the positive rate of DNER protein was associated with tumor diameter(χ^2=6.121,P=0.026),TNM staging(P=0.030)and lymph node metastases(P=0.005).Results of RTPCR showed that DNER mRNA expression in TNBC tissues was 0.768±0.067,significantly higher than 0.487±0.041 in adjacent normal tissues(t=4.793,P<0.001).The expression of DNER mRNA in TNBC tissues was correlated with tumor diameter(F=8.392,P=0.001),TNM staging(t=5.178,P<0.001)and lymph node metastases(t=4.925,P<0.001).Conclusion The high expression of DNER protein and mRNA is closely related to the occurrence,development and metastasis of TNBC,so DNER may be a potential target for the diagnosis and treatment of TNBC.