郁金根乙醇提取液对KM小鼠慢性肝损伤的研究

Study on chronic liver injury of KM mice by ethanol extracting solution of turmeric root

目的:探究郁金根乙醇提取液对慢性肝损伤小鼠的影响。方法:选取72只雄性小鼠,随机分成6组,每组12只,分别标记为空白组、模型组、阳性对照组、郁金高浓度组、郁金中浓度组、郁金低浓度组。除了空白组外各组用4%的四氯化碳(CCl4)制造慢性肝损伤模型。按照以上分组分别服用0.9%NaCl、0.9%NaCl、0.02 g/ml的Fenofibrater溶液、4 g/ml郁金根提取液、2 g/ml郁金根提取液、1 g/ml郁金根提取液,按小鼠的体重计算灌胃量(10 ml/kg),1次/d,连续灌胃14 d。用谷草和谷丙转氨酶试剂盒测小鼠血清AST/ALT、Western blot测小鼠肝脏MAPK信号通路中的p38的表达情况,并对组间数据进行对比。结果:与空白组相比,模型组的AST/ALT活性、p38的表达量显著升高,差异有统计学意义(P<0.05),郁金高、低浓度组的AST/ALT活性、p38的表达量降低,差异有统计学意义(P<0.05),郁金中浓度组的AST/ALT活性降低,差异有统计学意义(P<0.05);与模型组相比,阳性对照组、郁金高、中、低浓度组的AST/ALT活性、p38的表达量降低,差异有统计学意义(P<0.05);与阳性对照组相比,郁金高、中、低浓度组的AST/ALT活性、p38的表达量降低,差异有统计学意义(P<0.05)。结论:郁金根乙醇提取液对慢性肝损伤小鼠的治疗机制可能与降低血清AST/ALT和肝脏MAPK信号通路中p38的下调有关。

Objective To investigate the therapeutic mechanism of turmeric root ethanol extract on mice with chronic liver injury.Method 72 male mice were randomly divided into 6 groups,12 rats in each group,which were labeled as blank group,model group,positive control group,turmeric high concentration group,turmeric middle concentration group and turmeric low concentration group.In addition to the blank group,each group used 4%CCl4 to make a chronic liver injury model.According to the above group,0.9%NaCl,0.9%NaCl,0.02 g/ml Fenofibrater solution,4 g/ml turmeric root extract,2 g/ml turmeric root extract,1 g/ml turmeric root extract were used,and the stomach weight was calculated according to the weight of the mice.The amount(10 ml·kg-1),once/d,was continuously administered for 14 days.The expression of p38 in mouse liver MAPK signaling pathway was measured by AST/ALT and Western blot in mouse serum and alanine aminotransferase kit,and the data between groups were compared.Results Compared with the blank group,AST/ALT activity and p38 expression in the model group were significantly increased(P<0.05),AST/ALT activity and p38 expression in the high and low concentration groups were decreased(P<0.05),and AST/ALT activity in the medium concentration group was decreased(P<0.05).Compared with the model group,AST/ALT activity and p38 expression were decreased in the positive control group and the high,medium and low concentration group(P<0.05).Compared with the positive control group,AST/ALT activity and p38 expression levels were decreased in the high,medium and low concentration groups(P<0.05).Conclusion The therapeutic mechanism of turmeric root ethanol extract in mice with chronic liver injury may be related to the reduction of p38 in AST/ALT and MAPK signaling pathways.