4%吡丙醚·氟虫腈微囊悬浮-悬浮剂的高效液相色谱分析方法

Analysis of Pyriproxyfen+Fipronil 4% ZC by HPLC

建立一种同时分析吡丙醚和氟虫腈的高效液相色谱法。采用Shim-pack VP-ODS C18色谱柱和紫外-可见检测器,以乙腈+水为流动相,在278 nm波长下对4%吡丙醚·氟虫腈微囊悬浮-悬浮剂进行分离和定量分析。吡丙醚和氟虫腈的线性相关系数均为0.9999,标准偏差均为0.01,变异系数分别为0.87%、0.32%,平均回收率分别为100.02%、99.57%。结果表明,高效液相色谱方法灵敏、快速,适用于4%吡丙醚·氟虫腈微囊悬浮-悬浮剂的含量检测。

A HPLC method was developed for separation and quantitative analysis of pyriproxyfen and fipronil.The quantitative analysis of pyriproxyfen+fipronil 4%ZC was conducted with the mixture of acetonitrile and water as mobile phase,and with Shim-pack VP-ODS C18 column and UV-absorbance detector at 278 nm.The linear correlation coefficients of pyriproxyfen and fipronil were both 0.9999,the standard deviations were both 0.01,the variation coefficients were 0.87%and 0.32%,the average recoveries rates were 100.02%and 99.57%,respectively.In conclusion,the method was simple,quick,and suitable for analysis of pyriproxyfen+fipronil 4%ZC.