超高效液相色谱-串联质谱检测桃中吡唑醚菌酯和啶酰菌胺残留和消解

Determination of Pyraclostrobin and Boscalid Residue and Dissipation Dynamics in Peaches Using UPLC-MS/MS

本文建立了桃中吡唑醚菌酯和啶酰菌胺的超高效液相色谱-串联三重四极杆质谱(UPLC-MS/MS)检测方法,进行田间试验,明确了吡唑醚菌酯和啶酰菌胺在桃上的消解动态。结果表明,在添加水平0.01~2 mg/kg范围内,吡唑醚菌酯在桃中的平均回收率为89.8%~96.9%,相对标准偏差为0.8%~5.4%;啶酰菌胺的平均回收率为81.1%~100.5%,相对标准偏差为3.3%~12.1%;定量限均为0.01 mg/kg。吡唑醚菌酯和啶酰菌胺在桃中的消解动态均符合一级动力学方程,消解半衰期分别为7.2~15.8 d和7.0~11.8 d。

A method for the detection of pyraclostrobin and boscalid in peaches by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).A field experiment was conducted to determine the dissipa-tion dynamics of pyraclostrobin and boscalid on peaches.The results showed that average recoveries of pyraclostrobin ranged from 89.8%to 96.9%with the relative standard deviations of 0.8%to 5.4%.The average recoveries of boscalid ranged from 81.1%to 100.5%with the relative standard deviations of 3.3%to 12.1%at spiked levels(0.01 to 2 mg/kg).The limit of quantifications were 0.01 mg/kg.The dissipation equations of pyraclostrobin and boscalid in peach samples fit the first order kinetics.The half-lives of pyraclostrobin were 7.2 to 15.8 days and the half-lives of boscalid were 7.0 to 11.8 days.