高压脉冲电场辅助水酶法提取杜仲叶中不可萃取多酚

High-pressure Pulsed Electric Field Assisted Aqueous Enzymatic Extraction of Non-extractable Polyphenols from Eucommia ulmoides Leaves

为优化杜仲叶中不可萃取多酚(Non-extractable polyphenols,NEPP)提取工艺,选取酶用量、场强和脉冲数三个因素,在单因素的基础上,通过三因素三水平的响应面法对提取工艺进行优化,得出的最佳提取工艺为:酶用量18 mg,场强2.81 kV/cm,脉冲数100次,预测值为34.969 mg/g。在此条件下进行试验验证,不可萃取多酚的含量为(34.961±0.052)mg/g。

In order to optimize the non-extractable polyphenols( NEPP) extraction process in Eucommia ulmoides leaves,three factors including enzyme dosage,field strength and pulse number were selected. On the basis of single factor experiments,the extraction process was optimized by three-factor and three-level response surface method,and the best extraction was obtained.The process was as follows: Enzyme dosage 18 mg,field strength 2.81 k V/cm,pulse number 100 times,predicted value34.969 mg/g. The test was carried out under these conditions,and the contents of non-extractable polyphenol was( 34.961 ±0.952) mg/g.