液相色谱-串联质谱法测定进出口人参、西洋参中蚜灭磷等6种农药

Determination of 6 Pesticides in Imported and Exported Ginseng and Quinquefolium by High Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了人参、西洋参中凝胶色谱-固相萃取-液相色谱-串联质谱法测定蚜灭磷、吡虫啉、涕灭威、甲基内吸磷、甲硫威、辛硫磷6种农药的分析方法。样品经丙酮超声提取,凝胶色谱(GPC)净化后,固相萃取(SPE)净化,在HPLC/MS/MS多反应监测(MRM)模式下进行了定性和定量分析方法。在上述检测方法条件下,所测的6种农药的最低检出限为0.01 mg/kg,回收率范围为79.2%~107.3%,线性范围为0.01~0.2 mg/kg。

An analytical method has been established for the determination of six pesticides:aphid,imidacloprid,aldicarb,methyl endophosphate,methyl thiourea and phoxim in ginseng as well as quinquefolium by gel permeation chromatography-solid phase extraction-liquid chromatography-tandem mass spectrometry.The samples were extracted by acetone ultrasonic extraction,purified by gel permeation chromatography(GPC)and solid phase extraction(SPE)next.Qualitative and quantitative analysis were carried out under the HPLC/MS/MS multi reaction monitoring(MRM)mode.Under the above analysis conditions,the minimum detection limit of the six pesticides was 0.01 mg/kg,the recovery rate was 79.2%~107.3%,and the linear range was 0.01~0.2 mg/kg.