CK5/6、DSG3、P40、TTF-1、CK7、NapsinA在非小细胞肺癌组织中的表达及意义

Expression and clinical significance of CK5/6,DSG3,P40,TTF-1,CK7,NapsinA in small biopsy specimens of non-small cell lung cancer

目的探讨CK5/6、DSG3、P40、TTF-1、CK7、NapsinA在非小细胞肺癌(NSCLC)活检小标本中鳞状细胞癌(SCC)与腺癌(AC)的表达及其鉴别意义。方法采用免疫组化SP法检测2016年1月至2017年12月在滕州市中心人民医院住院治疗的非小细胞肺癌(NSCLC)120例活检小标本中CK5/6、DSG3、P40、TTF-1、CK7、NapsinA表达,并结合NSCLC的临床特征进行分析。结果CK5/6、DSG3和P40在肺鳞状细胞癌(SCC)组织中的表达率分别为100.0%(56/56)、89.3%(50/56)和96.4%(54/56),特异度分别为90.6%、100.0%和100.0%;NapsinA、TTF-1、CK7在肺腺癌(AC)组织中的表达率分别为81.3%(52/64)、90.6%(58/64)和93.8%(60/64),特异度分别为100.0%、92.9%和96.4%。CK5/6、DSG3和P40在SCC组织中的表达与在AC组织中的表达差异有统计学意义(P<0.05),NapsinA、TTF-1、CK7在AC组织中的表达较SCC组织中的表达差异有统计学意义(P<0.05)。结论CK5/6、DSG3、P40、TTF-1、CK7、NapsinA在非小细胞肺癌活检小标本SCC与AC的鉴别诊断中具有重要意义。

Objective To detect the expression and the differential significance of CK5/6,DSG3,P40,TTF-1,CK7,NapsinA in small biopsy specimens of non-small cell lung cancer(NSCLC),squamous cell carcinoma(SCC)and adenocarcinoma(AC).Methods Immunohistochemical SP method was used to detect the expressions of CK5/6,DSG3,P40,TTF-1,CK7 and NapsinA in 120 small biopsy specimens of NSCLC hospitalized in the Central People's Hospital of Tengzhou from January 2016 to December 2017,and the results were analyzed combined with the clinical characteristics of NSCLC.Results The positive expression rates of CK5/6,DSG3 and P40 in lung SCC were 100.0%(56/56),89.3%(50/56)and 96.4%(54/56),respectively,with specificity of 90.6%,100.0%and 100.0%,respectively.The positive expression rates of NapsinA,TTF-1 and CK7 in lung AC were 81.3%(52/64),90.6%(58/64)and 93.8%(60/64),respectively,with specificity of 100.0%,92.9%and 96.4%,respectively.The positive expression rates of CK5/6,DSG3,P40 in SCC had statistically significant differences compared with those in AC(all P<0.05),and the expression of TTF-1,CK7 and NapsinA in AC had statistically significant differences compared with those in SCC(all P<0.05).Conclusion CK5/6,DSG3,P40,TTF-1,CK7 and NapsinA are of great significance in the differential diagnosis of SCC and AC in small biopsy specimens of NSCLC.