摘要
疫苗是控制传染病最有效的措施。反向疫苗学是利用生物信息学方法,从基因组着手预测可能的疫苗候选抗原基因,对筛选出的候选抗原基因进行高通量克隆、表达,纯化出重组蛋白,再通过实验研究筛选出具有保护性的抗原作为疫苗。该文对反向疫苗学的基本研究方法以及在细菌、病毒、寄生虫疫苗研发中的应用进展进行综述。
Vaccines are the most effective way to control infectious diseases.Reverse vaccinology is the subject that uses bioinformatics to predict potential vaccine candidate antigen genes from genome,and clone and express candidate antigen genes in high throughput,purify recombinant proteins,and then screen protective antigens as vaccines through experimental research.This review is intended to introduce the basic research methods of reverse vaccinology and its application and progress in the research and development of bacterial,viral and parasitic vaccines.
作者
刘颖
张雷
LIU Ying;ZHANG Lei(Laboratory of Pathogenic Biology,School of Basic Medical Sciences,Dali University,Dali 671000,China)
出处
《生物技术》
CAS
2019年第6期610-615,共6页
Biotechnology
基金
国家自然科学基金项目(“基于全基因组序列的淋球菌候选疫苗筛选与鉴定”，81760303)
大理大学临床分子免疫学创新团队(ZKLX2019105)
